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https://hdl.handle.net/1822/9608
Título: | BC nanofibres: in vitro study of genotoxicity and cell proliferation |
Autor(es): | Moreira, Susana Margarida Gomes Silva, Naisandra Bezerra Almeida-Lima, Jailma Rocha, Hugo Alexandre Oliveira Medeiros, Silvia Regina Batistuzzo Alves Junior, Clodomiro Gama, F. M. |
Palavras-chave: | Nanofibres Nonotoxicology Bacterial cellulose Genotoxicity |
Data: | Set-2009 |
Editora: | Elsevier 1 |
Revista: | Toxicology Letters |
Citação: | "Toxicology Letters". ISSN 0378-4274. 189:3 (Sept. 2008) 235-241. |
Resumo(s): | Nanomaterials have unusual properties not found in the bulk materials, which can be exploited in numerous applications such as biosensing, electronics, scaffolds for tissue engineering, diagnostics and drug delivery. However, research in the past few years has turned up a range of potential health hazards, which has given birth to the new discipline of nanotoxicology. Bacterial cellulose (BC) is a promising material for biomedical applications, namely due its biocompatibility. Although BC has been shown not to be cytotoxic or genotoxic, the properties of isolated BC nanofibres (NFs) on cells and tissues has never been analysed. Considering the toxicity associated to other fibre-shaped nanoparticles, it seems crucial to evaluate the toxicity associated to the BC-NFs. In thiswork, nanofibreswere produced frombacterial cellulose by a combination of acid and ultrasonic treatment. The genotoxicity of nanofibres from bacterial cellulosewas analysed in vitro, using techniques previously demonstrated to detect the genotoxicity of fibrous nanoparticles. The results from single cell gel electrophoresis (also known as comet assay) and the Salmonella reversion assays showed that NFs are not genotoxicity under the conditions tested. A proliferation assay using fibroblasts and CHO cells reveals a slight reduction in the proliferation rate, although no modification in the cell morphology is observed. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/9608 |
DOI: | 10.1016/j.toxlet.2009.06.849 |
ISSN: | 0378-4274 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Moreira-TL[1].pdf | 1,22 MB | Adobe PDF | Ver/Abrir |