Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/9494
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Santos, Andréa F. S. | - |
dc.contributor.author | Luz, Luciana A. | - |
dc.contributor.author | Argôlo, Adriana C. C. | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Paiva, Patrícia M. G. | - |
dc.contributor.author | Coelho, L. C. B. B. | - |
dc.date.accessioned | 2009-08-17T15:52:42Z | - |
dc.date.available | 2009-08-17T15:52:42Z | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | "Process Biochemistry". ISSN 1359-5113. 44:4 (Apr. 2009) 504-508. | por |
dc.identifier.issn | 1359-5113 | por |
dc.identifier.uri | https://hdl.handle.net/1822/9494 | - |
dc.description.abstract | In this work hemagglutinating activity (HA) was investigated in distinct Moringa oleifera tissue extracts. A new lectin from seeds (cMoL) was purified and characterized; hemagglutinating and coagulating activities were evaluated. HA was detected in 0.15 M NaCl extracts from flowers and rachis inflorescence (5%, w/v), seeds, leaves, fundamental tissue of stem and steam bark (10%, w/v). cMoL isolated after saline extraction and guar gel column chromatography was active at pH range 4.0–9.0 agglutinating erythrocytes from rabbit and human blood types. Extracts of tissues and cMoL activities were carbohydrate inhibited; azocasein and asialofetuin abolished cMoL HA. The lectin was thermostable at 100 °C during 7 h. Polyacrylamide gel electrophoresis under reduced conditions revealed a main polypeptide band of 26.5 kDa; native basic cMoL was detected as a unique band. Seed lectin preparations and cMoL showed coagulant activity, similar to aluminium sulphate, the coagulant most widely used in water treatment. | por |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | por |
dc.description.sponsorship | Científico e Tecnológico (CNPq) | por |
dc.description.sponsorship | Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) | por |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Protein purification | por |
dc.subject | Lectin | por |
dc.subject | Moringa oleifera | por |
dc.subject | Coagulant activity | por |
dc.subject | Water treatment | por |
dc.subject | Natural coagulants | por |
dc.title | Isolation of a seed coagulant Moringa oleifera lectin | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.number | 4 | por |
sdum.pagination | 504-508 | por |
sdum.publicationstatus | published | por |
sdum.volume | 44 | por |
oaire.citationStartPage | 504 | por |
oaire.citationEndPage | 508 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 44 | por |
dc.identifier.doi | 10.1016/j.procbio.2009.01.002 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Process Biochemistry | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Santos_PB.pdf | 552,4 kB | Adobe PDF | Ver/Abrir |