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https://hdl.handle.net/1822/92880
Título: | Synthesis of phenylalanine/manganese-copper supramolecular sheets with laccase-like activity for the detection of phenolic pollutants |
Autor(es): | Chen, Xinyi Yang, Xue Su, Xiaolei Cavaco-Paulo, Artur Su, Jing Wang, Hongbo |
Palavras-chave: | Laccase-like Mn-F-Cu sheets Self-assembly Detection Phenolic pollutants |
Data: | Nov-2024 |
Editora: | Elsevier B.V. |
Revista: | Microchemical Journal |
Citação: | Chen, Xinyi; Yang, Xue; Su, Xiaolei; Cavaco-Paulo, Artur; Jing Su; Wang, Hongbo, Synthesis of phenylalanine/manganese-copper supramolecular sheets with laccase-like activity for the detection of phenolic pollutants. Microchemical Journal, 206(111380), 2024 |
Resumo(s): | Enzyme mimics with laccase-like activity showing excellent stability and tolerance have attracted extensive interest in phenolic pollutants treatment. Herein, we presented a mild and convenient strategy for the preparation of laccase-like sheets (denoted as Mn-F-Cu) inspired by the supramolecular self-assembly of the transition metals manganese, copper, and L-phenylalanine (F) under room temperature, alkaline conditions. The Mn-F-Cu sheets perform a better substrate affinity (Michaelis constant, Km = 0.65 mM) and a higher reaction rate (Vmax = 3.98 × 103 mM·min1) at 25 °C, pH 6.8 compared to natural laccase (Km = 1.08 mM; Vmax = 6.93 × 106 mM·min1). Besides, the catalytic stability data show they also exhibit good tolerance under alkaline pH (pH 810) and high temperature (7090 °C). More importantly, the prepared Mn-F-Cu material shows a relatively better sensitivity in detecting phenolic pollutants 2,4-DP and catechol than other laccase mimics mentioned in the literature, with a detection limit of 2.41 M and 3.49 M, respectively(R2 > 0.99). Therefore, Mn-F-Cu sheets show great potential to replace natural laccase for the detection and treatment of phenolic pollutants in harsh environments. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/92880 |
DOI: | 10.1016/j.microc.2024.111380 |
ISSN: | 0026-265X |
e-ISSN: | 1095-9149 |
Versão da editora: | https://www.journals.elsevier.com/microchemical-journal |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57964_1.pdf Acesso restrito! | 5,26 MB | Adobe PDF | Ver/Abrir |