Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/92724
Título: | Fucoidan/chitosan nanoparticles functionalized with anti-ErbB-2 target breast cancer cells and impair tumor growth in vivo |
Autor(es): | Oliveira, Catarina Gonçalves, Céline S. Martins, Eduarda P. Neves, N. M. Reis, R. L. Costa, Bruno M. Silva, Tiago H. Martins, Albino |
Palavras-chave: | Breast cancer Marine polymeric nanoparticles Targeting |
Data: | Mai-2021 |
Editora: | Elsevier B.V. |
Revista: | International Journal of Pharmaceutics |
Citação: | Oliveira C., Gonçalves C. S., Martins E. P., Neves N. M., Reis R. L., Costa B. M., Silva T. H., Martins A. Fucoidan/chitosan nanoparticles functionalized with anti-ErbB-2 target breast cancer cells and impair tumor growth in vivo, International Journal of Pharmaceutics, Vol. 600, pp. 120548, doi:10.1016/j.ijpharm.2021.120548, 2021 |
Resumo(s): | The work herein presented reports the development of fucoidan/chitosan nanoparticles (NPs) loaded with gemcitabine and functionalized with ErbB-2 antibody at their surface (NPs + Gem + Ab). The maximum immobilization of ErbB-2 on NPs' surface was set at 10 μg mL-1 and resulted in NPs with a size around 160 nm, a polydispersity index of 0.18, and a zeta potential of 21 mV. ErbB-2 is overexpressed in some subtypes of breast cancers, and the targeting capability of the NPs + Gem + Ab system was confirmed by an increased cellular uptake of SKBR3 cells (ErbB-2 positive) when compared to MDA-MB-231 (ErbB-2 negative). To validate the targeting efficacy of NPs + Gem + Ab, a co-culture system with human endothelial and SKBR3 cells was established. Cytotoxic effects over endothelial cells were similar for all the tested conditions (between 25 and 30%). However, the NPs + Gem + Ab system presented increased toxicity over breast cancer cells, above 80% after 24 h, when compared to free Gem and NPs + Gem (around 15% and 20%, respectively). In vivo studies demonstrated that the developed targeting system significantly reduced tumor growth and the appearance of lung metastasis compared to untreated controls. In summary, the efficacy of the NPs + Gem + Ab system to target cancer cells was established and validated both in vitro and in vivo, being a compelling alternative strategy to current chemotherapeutic approaches. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/92724 |
DOI: | 10.1016/j.ijpharm.2021.120548 |
ISSN: | 0378-5173 |
e-ISSN: | 1873-3476 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0378517321003537?via%3Dihub |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20562-1-s20-s0378517321003537-main-2.pdf Acesso restrito! | 7,24 MB | Adobe PDF | Ver/Abrir |