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https://hdl.handle.net/1822/92685
Título: | Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol |
Autor(es): | Magalhães, C. P. Alves, Joana I. Duber, Anna Oleskowicz-Popiel, Piotr Stams, Alfons Johannes Maria Cavaleiro, Ana Júlia |
Palavras-chave: | Acetate Glycerol Metabolism Methane Microbial communities Propionate |
Data: | 2024 |
Editora: | Springer Nature |
Revista: | Applied Microbiology and Biotechnology |
Citação: | Magalhães, C.P., Alves, J.I., Duber, A. et al. Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol. Appl Microbiol Biotechnol 108, 419 (2024). https://doi.org/10.1007/s00253-024-13248-6 |
Resumo(s): | Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste glycerol valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three distinct microbial cultures were obtained from the same inoculum (anaerobic granular sludge), with high microbial specialization, within three different phyla (Thermodesulfobacteriota, Euryarchaeota and Pseudomonadota). The cultures are capable of glycerol conversion through different pathways: (i) glycerol conversion to methane by a bacterium closely related to Solidesulfovibrio alcoholivorans (99.8% 16S rRNA gene identity), in syntrophic relationship with Methanofollis liminatans (98.8% identity), (ii) fermentation to propionate by Propionivibrio pelophilus strain asp66 (98.6% identity), with a propionate yield of 0.88 mmol mmol1 (0.71 mg mg1) and a propionate purity of 8097% and (iii) acetate production coupled to sulfate reduction by Desulfolutivibrio sulfoxidireducens (98.3% identity). In conclusion, starting from the same inoculum, we could drive the metabolic and functional potential of the microbiota towards the formation of several valuable products that can be used in industrial applications or as energy carriers. Key points: Versatility of anaerobic cultures was explored for waste glycerol valorization Different environmental conditions lead to metabolic specialization Biocommodities such as propionate, acetate and methane were produced. |
Tipo: | Artigo |
Descrição: | The online version contains supplementary material available at https://doi.org/10.1007/s00253-024-13248-6 |
URI: | https://hdl.handle.net/1822/92685 |
DOI: | 10.1007/s00253-024-13248-6 |
ISSN: | 0175-7598 |
Versão da editora: | https://link.springer.com/article/10.1007/s00253-024-13248-6 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57943_1.pdf | 1,16 MB | Adobe PDF | Ver/Abrir |