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https://hdl.handle.net/1822/92450
Título: | The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cytochrome c oxidase inhibition |
Autor(es): | Mendes, Filipa Diana Silva Santos-Pereira, Cátia Vieira, Tatiana F. Pinto, Mélanie Martins Castro, Bruno B. Sousa, Sérgio F. Sousa, Maria João Devin, Anne Chaves, Susana Alexandra Rodrigues |
Palavras-chave: | Cymoxanil Cytochrome c Cytochrome c oxidase Mode of action Respiration |
Data: | Jul-2024 |
Editora: | Wiley-Blackwell |
Revista: | FEBS Letters |
Citação: | Mendes, Filipa; Cátia S. Pereira; Vieira, Tatiana F.; Martins Pinto, Mélanie; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria João; Devin, Anne; Chaves, Susana R., The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cytochrome c oxidase inhibition. FEBS Letters, 598(13), 1655-1666, 2024 |
Resumo(s): | Cymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase (CcO) activity during oxidative phosphorylation. Based on molecular docking, we propose that CYM blocks the interaction of cytochrome c with CcO, hampering electron transfer and inhibiting CcO catalytic activity. Although other targets cannot be excluded, our data offer valuable insights into the mode of action of CYM that will be instrumental in driving informed management of the use of this fungicide. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/92450 |
DOI: | 10.1002/1873-3468.14907 |
ISSN: | 0014-5793 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_57857_1.pdf Acesso restrito! | 808,61 kB | Adobe PDF | Ver/Abrir |