Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/91947
Título: | Radiotherapy metastatic prostate cancer cell lines treated with gold nanorods modulate miRNA signatures |
Autor(es): | Soares, Sílvia Aires, Fátima Monteiro, Armanda Pinto, Gabriela Faria, Isabel Sales, Goreti Correa-Duarte, Miguel A. Guerreiro, Susana Fernandes, Rúben |
Palavras-chave: | Gold nanorods Radiotherapy MicroRNAs Prostate cancer cell lines |
Data: | 27-Fev-2024 |
Editora: | MDPI |
Revista: | International Journal of Molecular Sciences |
Citação: | Soares, S.; Aires, F.; Monteiro, A.; Pinto, G.; Faria, I.; Sales, G.; Correa-Duarte, M.A.; Guerreiro, S.; Fernandes, R. Radiotherapy Metastatic Prostate Cancer Cell Lines Treated with Gold Nanorods Modulate miRNA Signatures. Int. J. Mol. Sci. 2024, 25, 2754. https://doi.org/10.3390/ijms25052754 |
Resumo(s): | MicroRNA (miRNA) modulation has been identified as a promising strategy for improving the response of human prostate cancer (PCa) to radiotherapy (RT). Studies have shown that mimics or inhibitors of miRNAs could modulate the sensitivity of PCa cells to RT. In addition, pegylated gold nanoparticles have been studied as a therapeutic approach to treat PCa cells and/or vehicles for carrying miRNAs to the inside of cells. Therefore, we evaluated the capacity of hypofractionated RT and pegylated gold nanorods (AuNPr-PEG) to modulate the miRNA signature on PCa cells. Thus, RT-qPCR was used to analyze miRNA-95, miRNA-106-5p, miRNA-145-5p, and miRNA-541-3p on three human metastatic prostate cell lines (PC3, DU145, and LNCaP) and one human prostate epithelial cell line (HprEpiC, a non-tumor cell line) with and without treatment. Our results showed that miRNA expression levels depend on cell type and the treatment combination applied using RT and AuNPr-PEG. In addition, cells pre-treated with AuNPr-PEG and submitted to 2.5 Gy per day for 3 days decreased the expression levels of miRNA-95, miRNA-106, miRNA-145, and miRNA-541-3p. In conclusion, PCa patients submitted to hypofractionated RT could receive personalized treatment based on their metastatic cellular miRNA signature, and AuNPr-PEG could be used to increase metastatic cell radiosensitivity. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/91947 |
ISBN: | 1424-6783 |
DOI: | 10.3390/ijms25052754 |
e-ISSN: | 1422-0067 |
Versão da editora: | https://www.mdpi.com/1422-0067/25/5/2754 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57763_1.pdf | 1,71 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons