Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/90808
Título: | Measuring DNA modifications with the comet assay: a compendium of protocols |
Autor(es): | Collins, Andrew Moller, Peter Gajski, Goran Vodenkova, Sona Abdulwahed, Abdulhadi Anderson, Diana Bankoglu, Ezgi Eyluel Bonassi, Stefano Boutet-Robinet, Elisa Brunborg, Gunnar Chao, Christy Cooke, Marcus S. S. Costa, Carla Costa, Solange Dhawan, Alok de Lapuente, Joaquin Del Bo, Cristian Dubus, Julien Dusinska, Maria Duthie, Susan J. J. El Yamani, Naouale Engelward, Bevin Gaivao, Isabel Giovannelli, Lisa Godschalk, Roger Guilherme, Sofia Gutzkow, Kristine B. B. Habas, Khaled Hernandez, Alba Herrero, Oscar Isidori, Marina Jha, Awadhesh N. N. Knasmueller, Siegfried Kooter, Ingeborg M. M. Koppen, Gudrun Kruszewski, Marcin Ladeira, Carina Laffon, Blanca Larramendy, Marcelo Le Hegarat, Ludovic Lewies, Angelique Lewinska, Anna Liwszyc, Guillermo E. E. de Cerain, Adela Lopez Manjanatha, Mugimane Marcos, Ricard Milic, Mirta de Andrade, Vanessa Moraes Moretti, Massimo Muruzabal, Damian Novak, Matjaz Oliveira, Rui Pedro Soares de Olsen, Ann-Karin Owiti, Norah Pacheco, Mario Pandey, Alok K. K. Pfuhler, Stefan Pourrut, Bertrand Reisinger, Kerstin Rojas, Emilio Runden-Pran, Elise Sanz-Serrano, Julen Shaposhnikov, Sergey Sipinen, Ville Smeets, Karen Stopper, Helga Teixeira, Joao Paulo Valdiglesias, Vanessa Valverde, Mahara van Acker, Frederique van Schooten, Frederik-Jan Vasquez, Marie Wentzel, Johannes F. F. Wnuk, Maciej Wouters, Annelies Zegura, Bojana Zikmund, Tomas Langie, Sabine A. S. Azqueta, Amaya |
Data: | 27-Jan-2023 |
Editora: | Nature Research |
Revista: | Nature Protocols |
Citação: | Collins, A., Møller, P., Gajski, G. et al. Measuring DNA modifications with the comet assay: a compendium of protocols. Nat Protoc 18, 929–989 (2023). https://doi.org/10.1038/s41596-022-00754-y |
Resumo(s): | The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions. |
Tipo: | Artigo |
Descrição: | Supplementary information The online version contains supplementary material available at https://doi.org/10.1038/s41596-022-00754-y |
URI: | https://hdl.handle.net/1822/90808 |
DOI: | 10.1038/s41596-022-00754-y |
ISSN: | 1754-2189 |
Versão da editora: | https://www.nature.com/articles/s41596-022-00754-y |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Collins et al., 2023_Nature Protocols.pdf Acesso restrito! | 4,07 MB | Adobe PDF | Ver/Abrir |