Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/89459
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Campo DC | Valor | Idioma |
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dc.contributor.author | Dias, Bruna | por |
dc.contributor.author | Lopes, Marlene | por |
dc.contributor.author | Fernandes, Helena | por |
dc.contributor.author | Marques, Susana | por |
dc.contributor.author | Gírio, Francisco | por |
dc.contributor.author | Belo, Isabel | por |
dc.date.accessioned | 2024-03-12T17:51:56Z | - |
dc.date.available | 2024-03-12T17:51:56Z | - |
dc.date.issued | 2024-04 | - |
dc.identifier.citation | Dias, B., Lopes, M., Fernandes, H., Marques, S., Gírio, F., & Belo, I. (2024, April). Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate. Renewable Energy. Elsevier BV. http://doi.org/10.1016/j.renene.2024.120173 | por |
dc.identifier.issn | 0960-1481 | por |
dc.identifier.uri | https://hdl.handle.net/1822/89459 | - |
dc.description.abstract | Using lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2gL1 CSL, 1.8gL1(NH4)2SO4, and kLa of 66 h1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6gL1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries. | por |
dc.description.sponsorship | This study was supported by the Move2LowC project (POCI-01- 0247-FEDER-046117), cofinanced by Programa Operacional Competitividade e Internacionalizaçao, Programa Operacional Regional de Lisboa, Portugal 2020 and the European Union, through the European Regional Development Fund. It was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit (DOI 10.54499/UIDB/04469/2020) and the Doctoral grant (2021.05799. BD). The authors gratefully acknowledge RAIZ (Forest and Paper Research Institute) for providing the eucalyptus bark material, and Novozymes A/S for supplying Cellic® CTec3 HS. The technical assistance of STEX company (Aveiro, Portugal) on the operation of pilot scale infrastructure of steam explosion and enzymatic hydrolysis is also acknowledged. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/2021.05799.BD/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | Eucalyptus bark hydrolysate | por |
dc.subject | Microbial lipids | por |
dc.subject | Volumetric oxygen transfer coefficient | por |
dc.subject | Yarrowia lipolytica | por |
dc.title | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0960148124002386 | por |
dc.comments | CEB57607 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 224 | por |
dc.date.updated | 2024-03-09T10:29:27Z | - |
dc.identifier.eissn | 1879-0682 | por |
dc.identifier.doi | 10.1016/j.renene.2024.120173 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Renewable Energy | por |
oaire.version | VoR | por |
dc.identifier.articlenumber | 120173 | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57607_1.pdf | 969,4 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons