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https://hdl.handle.net/1822/89371
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Pedro Miguel Peixoto | por |
dc.contributor.author | Neto, Mafalda D | por |
dc.contributor.author | Cerqueira, Miguel Ângelo Parente Ribeiro | por |
dc.contributor.author | Rodriguez, Isabel | por |
dc.contributor.author | Bourbon, Ana Isabel Juncá Sottomayor Lisboa | por |
dc.contributor.author | Azevedo, Ana Gabriela | por |
dc.contributor.author | Pastrana, Lorenzo M | por |
dc.contributor.author | Coimbra, Manuel A | por |
dc.contributor.author | Vicente, A. A. | por |
dc.contributor.author | Gonçalves, Catarina | por |
dc.date.accessioned | 2024-03-07T21:20:59Z | - |
dc.date.available | 2024-03-07T21:20:59Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.citation | Silva, P. M., Neto, M. D., Cerqueira, M. A., Rodriguez, I., Bourbon, A. I., Azevedo, A. G., … Gonçalves, C. (2024, February). Resveratrol-loaded octenyl succinic anhydride modified starch emulsions and hydroxypropyl methylcellulose (HPMC) microparticles: Cytotoxicity and antioxidant bioactivity assessment after in vitro digestion. International Journal of Biological Macromolecules. Elsevier BV. http://doi.org/10.1016/j.ijbiomac.2024.129288 | por |
dc.identifier.issn | 0141-8130 | por |
dc.identifier.uri | https://hdl.handle.net/1822/89371 | - |
dc.description.abstract | Hydroxypropyl methylcellulose (HPMC)-based microparticles and modified starch emulsions (OSA-MS) were loaded with resveratrol and characterized regarding their physicochemical and thermal properties. Both delivery systems were subject to an in vitro gastrointestinal digestion to assess the bioaccessibility of resveratrol. In addition, cell-based studies were conducted after in vitro digestion and cytotoxicity and oxidative stress were assessed. HPMC-based microparticles displayed higher average sizes (d) and lower polydispersity index (PDI) (d = 948 nm, PDI < 0.2) when compared to OSA-MS-based emulsions (d = 217 nm, PDI < 0.3). Both proved to protect resveratrol under digestive conditions, leading to an increase in bioaccessibility. Resveratrol-loaded HPMC-microparticles showed a higher bioaccessibility (56.7 %) than resveratrol-loaded emulsions (19.7 %). Digested samples were tested in differentiated co-cultures of Caco-2 and HT29-MTX, aiming at assessing cytotoxicity and oxidative stress, and a lack of cytotoxicity was observed for all samples. Results displayed an increasing antioxidant activity, with 1.6-fold and 1.4-fold increases over the antioxidant activity of free resveratrol, for HPMC-microparticles and OSA-MS nanoemulsions, respectively. Our results offer insight into physiological relevancy due to assessment post-digestion and highlight the protection that the use of micro-nano delivery systems can confer to resveratrol and their potential to be used as functional food ingredients capable of providing antioxidant benefits upon consumption. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020 and LAQV/REQUIMTE (UIDB/50006/2020, UIDP/50006/2020). This work was funded by the SbDtoolBox - Nanotechnology-based tools and tests for Safer-by-Design nanomaterials, with the reference NORTE-01-0145-FEDER-000047, funded by Norte 2020 – North-Regional Operational Program under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). The research also received funding from the European Union's H2020 research and innovation program under the Marie Sklodowska-Curie grant agreement N 778388 (H2020 MSCA-RISE-2017 project Food for Diabetes and Cognition (FODIAC), and grant agreement N 713640 (MSCA-2015-COFUND-FP). Pedro Silva is the recipient of a fellowship (SFRD/BD/130247/2017) supported by Fundação para a Ciência e a Tecnologia, (FCT, Portugal). Ana Isabel Bourbon acknowledges funding by FCT, through the individual scientific employment program contract (2020.03447.CEECIND). We also would like to thank the Advanced Electron Microscopy, Imaging, and Spectroscopy (AEMIS) and the Nanophotonics and Bioimaging Facility (NBI) from INL for their support. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/778388/EU | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/713640/EU | por |
dc.relation | 2020.03447.CEECIND | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Humans | por |
dc.subject | Emulsions | por |
dc.subject | Resveratrol | por |
dc.subject | Hypromellose Derivatives | por |
dc.subject | Caco-2 Cells | por |
dc.subject | Starch | por |
dc.subject | Digestion | por |
dc.subject | Antioxidants | por |
dc.subject | Succinic Anhydrides | por |
dc.subject | EHDP | por |
dc.subject | Functional foods | por |
dc.subject | Gastrointestinal fate | por |
dc.subject | Intracellular ROS | por |
dc.title | Resveratrol-loaded octenyl succinic anhydride modified starch emulsions and hydroxypropyl methylcellulose (HPMC) microparticles: Cytotoxicity and antioxidant bioactivity assessment after in vitro digestion | por |
dc.type | article | eng |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0141813024000916 | por |
oaire.citationVolume | 259 | por |
dc.identifier.doi | 10.1016/j.ijbiomac.2024.129288 | por |
dc.identifier.pmid | 38211926 | por |
sdum.journal | International Journal of Biological Macromolecules | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S0141813024000916-main.pdf | 2,96 MB | Adobe PDF | Ver/Abrir |
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