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https://hdl.handle.net/1822/88269
Título: | Microbial production of the building block p-Coumaric Acid: unleashing the potential of Kluyveromyces marxianus |
Autor(es): | Baptista, Marlene Isabel Cerqueira Montenegro Costa, Carlos E. Domingues, Lucília |
Palavras-chave: | Kluyveromyces marxianus p-coumaric acid Tyrosine ammonia lyase Precision fermentation |
Data: | 7-Set-2023 |
Editora: | Universidade da Beira Interior (UBI) |
Citação: | Baptista, Marlene; Costa, Carlos E.; Domingues, Lucília, Microbial Production of the building block p-Coumaric Acid: Unleashing the Potential of Kluyveromyces marxianus. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P5.15, Covilhã, Portugal, Dec 07-09, 497, 2023. |
Resumo(s): | The interest in several plant secondary metabolites, such as naringenin or resveratrol, for their known biological functions, is growing at a fast pace. These properties encompass antioxidant, antiinflammatory, and anti-microbial, among many other activities. The phenolic acid p-coumaric acid is naturally produced by plants, being a key precursor of many of these secondary metabolites. Nevertheless, p-coumaric acid is currently extracted from plants, which poses several drawbacks for its industrial production including low efficiency and dependence on plant availability. On the other hand, microbial production of p-coumaric acid has been emerging as a sustainable and economically viable alternative. The unconventional yeast Kluyveromyces marxianus is garnering increasing interest as an alternative cell platform to produce ethanol and high-value compounds with a span of applications across industries [1]. This is due to its distinctive attributes, such as rapid growth rate, thermotolerance, and the capacity to metabolize different sugars [2]. Due to its Crabtree-negative metabolism, this yeast produces acetyl-coenzyme A in the presence of oxygen and high sugar concentration. As such, it could be an interesting chassis to produce aromatic compounds derived from p-coumaric since some of them require malonyl-CoA (derived from acetyl-CoA) as a precursor. For that, in yeast, the expression of heterologous enzymes involved in the conversion of aromatic amino acids into p-coumaric acid is required. Building upon this knowledge, here, two K. marxianus strains were engineered and screened for their capacity for p-coumaric acid production. Initially, a heterologous enzyme, tyrosine ammonia lyase (TAL), which converts tyrosine to p-coumaric acid, was integrated into both strains. Further, the effects of different carbon sources and agitation conditions on p-coumaric acid production and yeast primary metabolism were evaluated. Overall, this work shows the potential of K. marxianus for p-coumaric acid production and its derivatives through an integrated process. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/88269 |
Versão da editora: | https://microbiotec23.organideia.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56577_1.pdf | 248,23 kB | Adobe PDF | Ver/Abrir |