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https://hdl.handle.net/1822/87228
Título: | Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage |
Autor(es): | da Silva, Jéssica Duarte Melo, Luís Daniel Rodrigues Santos, Sílvio Roberto Branco Kropinski, Andrew M. Xisto, Mariana Fonseca Dias, Roberto Sousa da Silva Paes, Isabela Vieira, Marcella Silva Soares, José Júnior Ferreira Porcellato, Davide da Silva Duarte, Vinícius de Paula, Sérgio Oliveira |
Palavras-chave: | Bovine mastitis Endolysin Jumbo phages Staphylococcus aureus VAPGH |
Data: | 2023 |
Editora: | Springer Nature |
Revista: | Applied Microbiology and Biotechnology |
Citação: | da Silva, Jéssica Duarte; Melo, Luís Daniel Rodrigues; Santos, Sílvio Roberto Branco; Kropinski, Andrew M.; Xisto, Mariana Fonseca; Dias, Roberto Sousa; da Silva Paes, Isabela; Vieira, Marcella Silva; Soares, José Júnior Ferreira; Porcellato, Davide; da Silva Duarte, Vinícius; de Paula, Sérgio Oliveira, Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage. Applied Microbiology and Biotechnology, 107, 7231-7250, 2023 |
Resumo(s): | Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. Among the different strategies to tackle S. aureus infection in livestock, bacteriophages have been thoroughly investigated in the last decades; however, few specimens of the so-called jumbo phages capable of infecting S. aureus have been described. Herein, we report the biological, genomic, and structural proteomic features of the jumbo phage vB_SauM-UFV_DC4 (DC4). DC4 exhibited a remarkable killing activity against S. aureus isolated from the veterinary environment and stability at alkaline conditions (pH 4 to 12). The complete genome of DC4 is 263,185 bp (GC content: 25%), encodes 263 predicted CDSs (80% without an assigned function), 1 tRNA (Phe-tRNA), multisubunit RNA polymerase, and an RNA-dependent DNA polymerase. Moreover, comparative analysis revealed that DC4 can be considered a new viral species belonging to a new genus DC4 and showed a similar set of lytic proteins and depolymerase activity with closely related jumbo phages. The characterization of a new S. aureus jumbo phage increases our understanding of the diversity of this group and provides insights into the biotechnological potential of these viruses. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/87228 |
DOI: | 10.1007/s00253-023-12743-6 |
ISSN: | 0175-7598 |
Versão da editora: | https://link.springer.com/article/10.1007/s00253-023-12743-6 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56442_1.pdf Acesso restrito! | 1,35 MB | Adobe PDF | Ver/Abrir |