Engineered Ashbya gossypii for single-cell oil production from non-detoxified Eucalyptus bark hydrolysate

Abstract

[Excerpt] Ashbya gossypii is a filamentous fungus industrially used for riboflavin production, a bioprocess in which downstream product recovery is facilitated by the ability of this fungus to undergo autolysis during the late stationary phase of growth or at low temperature [1]. In addition to riboflavin, engineered A. gossypii strains are capable of producing other compounds of interest for the food and feed industry, among which Single-Cell Oils (SCOs) from media containing mixed formulations of detoxified corn-cob hydrolysate, sugarcane molasses or crude glycerol [2]. [...]