Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/86388
Título: | Electrochemical miRNA-34a-based biosensor for the diagnosis of Alzheimer’s disease |
Autor(es): | Pereira, Raquel L. Oliveira, Daniela Pêgo, Ana P. Santos, Sofia D. Moreira, Felismina T. C. |
Palavras-chave: | miRNA-34a Electrochemical biosensor Alzheimer's disease Gold nanoparticles Carbon screen-printed electrodes |
Data: | Dez-2023 |
Editora: | Elsevier 1 |
Revista: | Bioelectrochemistry |
Citação: | Pereira, Raquel L.; Oliveira, Daniela; Pêgo, Ana P.; Santos, Sofia D.; Moreira, Felismina, Electrochemical miRNA-34a-based biosensor for the diagnosis of Alzheimers disease. Bioelectrochemistry, 154(108553), 2023 |
Resumo(s): | Alzheimer's disease (AD) is the most common dementia type and a leading cause of death and disability in the elderly. Diagnosis is expensive and invasive, urging the development of new, affordable, and less invasive diagnostic tools. The identification of changes in the expression of non-coding RNAs prompts the development of diagnostic tools to detect disease-specific blood biomarkers. Building on this idea, this work reports a novel electrochemical microRNA (miRNA) biosensor for the diagnosis of AD, based on carbon screen-printed electrodes (C-SPEs) modified with two gold nanostructures and a complementary anti-miR-34a oligonucleotide probe. This biosensor showed good target affinity, reflected on a 100 pM to 1 M linearity range and a limit of detection (LOD) of 39 pM in buffer and 94 aM in serum. Moreover, the biosensors response was not affected by serum compounds, indicating selectivity for miR-34a. The biosensor also detected miR-34a in the cell culture medium of a common AD model, stimulated with a neurotoxin to increase miR-34a secretion. Overall, the proposed biosensor makes a solid case for the introduction of a novel, inexpensive, and minimally invasive tool for the early diagnosis of AD, based on the detection of a circulating miRNA overexpressed in this pathology. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/86388 |
DOI: | 10.1016/j.bioelechem.2023.108553 |
ISSN: | 1567-5394 |
Versão da editora: | http://www.journals.elsevier.com/bioelectrochemistry/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56391_1.pdf | 5,54 MB | Adobe PDF | Ver/Abrir |