Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/86077
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Campo DC | Valor | Idioma |
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dc.contributor.author | Costa, Ana Rita | por |
dc.contributor.author | Fernandes, Helena | por |
dc.contributor.author | Salgado, José Manuel | por |
dc.contributor.author | Belo, Isabel | por |
dc.date.accessioned | 2023-08-09T21:08:48Z | - |
dc.date.available | 2023-08-09T21:08:48Z | - |
dc.date.issued | 2023-08-06 | - |
dc.identifier.citation | Costa, A.R.; Fernandes, H.; Salgado, J.M.; Belo, I. Solid State and Semi-Solid Fermentations of Olive and Sunflower Cakes with Yarrowia lipolytica: Impact of Biological and Physical Pretreatments. Fermentation 2023, 9, 734. https://doi.org/10.3390/fermentation9080734 | por |
dc.identifier.uri | https://hdl.handle.net/1822/86077 | - |
dc.description.abstract | Lignocellulosic biomass is a promising feedstock for added value compound production in biotechnological processes such as solid-state fermentation (SSF). Although these solid materials can be directly used as substrates in fermentations in a solid state, a pretreatment is often required, especially if the microorganism selected is unable to produce lignocellulosic enzymes. In the present work, several pretreatment strategies were applied to a 50% (w/w) mixture of olive and sunflower cakes before SSF for lipase production by the oleaginous yeast Yarrowia lipolytica W29. Co-culture strategies with Y. lipolytica and Aspergillus niger did not improve lipase production by the oleaginous yeast. Biological pretreatment with a fungal enzymatic extract led to a significant increase in sugar availability in the substrate mixture after a short incubation period, improving yeast growth. Microwave and ultrasound were the physical pretreatments selected and microwave irradiation proved to be the best method, resulting in 44% and 17% increases in yeast growth and lipase production, respectively, compared to the untreated mixture. An improvement in lipase activity was also observed after ultrasonic treatment in semi-solid fermentations, leading to a 2-fold increase in this enzyme activity compared to the control. The utilization of pretreatments before SSF with Y. lipolytica can increase sugars availability and result in structural changes in the solid substrate, which can improve the bioprocesses’ productivity. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 and the Ph.D grant SFRH/BD/139098/2018 and by LABBELS, Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F139098%2F2018/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Yarrowia lipolytica | por |
dc.subject | solid state fermentation | por |
dc.subject | sunflower cake | por |
dc.subject | olive cake | por |
dc.subject | lignocellulosic biomass pretreatment | por |
dc.title | Solid state and semi-solid fermentations of olive and sunflower cakes with Yarrowia lipolytica: impact of biological and physical pretreatments | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2311-5637/9/8/734 | por |
dc.comments | CEB56367 | por |
oaire.citationStartPage | 734 | por |
oaire.citationIssue | 8 | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2023-08-09T16:04:26Z | - |
dc.identifier.eissn | 2311-5637 | por |
dc.identifier.doi | 10.3390/fermentation9080734 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Fermentation | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56367_1.pdf | 2,12 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons