Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85564
Título: | A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities |
Autor(es): | Elnaggar, Jacob H. Ardizzone, Caleb M. Cerca, Nuno Toh, Evelyn Laniewski, Pawel Lillis, Rebecca A. Herbst-Kralovetz, Melissa M. Quayle, Alison J. Muzny, Christina A. Taylor, Christopher M. |
Palavras-chave: | bacterial burden vaginal microbiome bacterial vaginosis biofilm qPCR standard Gardnerella Prevotella Lactobacillus |
Data: | 19-Mar-2023 |
Editora: | Frontiers Media S.A. |
Revista: | Frontiers in Cellular and Infection Microbiology |
Citação: | Elnaggar, J. H., Ardizzone, C. M., Cerca, N., Toh, E., Łaniewski, P., Lillis, R. A., … Taylor, C. M. (2023, June 19). A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities. Frontiers in Cellular and Infection Microbiology. Frontiers Media SA. http://doi.org/10.3389/fcimb.2023.1198113 |
Resumo(s): | Bacterial vaginosis (BV) is the most common vaginal dysbiosis. In this condition, a polymicrobial biofilm develops on vaginal epithelial cells. Accurately quantifying the bacterial burden of the BV biofilm is necessary to further our understanding of BV pathogenesis. Historically, the standard for calculating total bacterial burden of the BV biofilm has been based on quantifying Escherichia coli 16S rRNA gene copy number. However, E. coli is improper for measuring the bacterial burden of this unique micro-environment. Here, we propose a novel qPCR standard to quantify bacterial burden in vaginal microbial communities, from an optimal state to a mature BV biofilm. These standards consist of different combinations of vaginal bacteria including three common BV-associated bacteria (BVAB) Gardnerella spp. (G), Prevotella spp. (P), and Fannyhessea spp. (F) and commensal Lactobacillus spp. (L) using the 16S rRNA gene (G:P:F:L, G:P:F, G:P:L and 1G:9L). We compared these standards to the traditional E. coli (E) reference standard using known quantities of mock vaginal communities and 16 vaginal samples from women. The E standard significantly underestimated the copy numbers of the mock communities, and this underestimation was significantly greater at lower copy numbers of these communities. The G:P:L standard was the most accurate across all mock communities and when compared to other mixed vaginal standards. Mixed vaginal standards were further validated with vaginal samples. This new G:P:L standard can be used in BV pathogenesis research to enhance reproducibility and reliability in quantitative measurements of BVAB, spanning from the optimal to non-optimal (including BV) vaginal microbiota. |
Tipo: | Artigo |
Descrição: | The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/full#supplementary-material |
URI: | https://hdl.handle.net/1822/85564 |
DOI: | 10.3389/fcimb.2023.1198113 |
ISSN: | 2235-2988 |
Versão da editora: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/full |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56342_1.pdf | 1,27 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons