Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85453
Título: | Starvation combined with constant anode potential triggers intracellular electron storage in electro-active biofilms |
Autor(es): | Pereira, João Neves, Patrícia Nemanic, Vivian Pereira, M. Alcina Sleutels, Tom Hamelers, Bert ter Heijne, Annemiek |
Palavras-chave: | electro-active biofilms intermittent anode potential feeding modes electron storage |
Data: | 15-Ago-2023 |
Editora: | Elsevier 1 |
Revista: | Water Research |
Citação: | Pereira, João; Neves, Patrícia; Nemanic, Vivian; Pereira, M. Alcina; Sleutels, Tom; Hamelers, Bert; ter Heijne, Annemiek, Starvation combined with constant anode potential triggers intracellular electron storage in electro-active biofilms. Water Research, 242(120278), 2023 |
Resumo(s): | The accumulation of electrons in the form of Extracellular Polymeric Substances (EPS) and poly-hydroxyalkanoates (PHA) has been studied in anaerobic processes by adjusting the access of microorganisms to the electron donor and final electron acceptor. In Bio-electrochemical systems (BESs), intermittent anode potential regimes have also recently been used to study electron storage in anodic electro-active biofilms (EABfs), but the effect of electron donor feeding mode on electron storage has not been explored. Therefore, in this study, the accumulation of electrons in the form of EPS and PHA was studied as a function of the operating conditions. EABfs were grown under both constant and intermittent anode potential regimes and fed with acetate (electron donor) continuously or in batch. Confocal Laser Scanning Microscopy (CLSM) and Fourier-Transform Infrared Spectroscopy (FTIR) were used to assess electron storage. The range of Coulombic efficiencies, from 25 to 82 %, and the biomass yields, between 10 and 20 %, indicate that storage could have been an alternative electron consuming process. From image processing, a 0.92 pixel ratio of poly-hydroxybutyrate (PHB) and amount of cells was found in the batch fed EABf grown under a constant anode potential. This storage was linked to the presence of living Geobacter and shows that energy gain and carbon source starvation were the triggers for intracellular electron storage. The highest EPS content (extracellular storage) was observed in the continuously fed EABf under an intermittent anode potential, showing that constant access to electron donor and intermittent access to the electron acceptor leads to the formation of EPS from the excess energy gained. Tailoring operating conditions can thus steer the microbial community and result in a trained EABf to perform a desired biological conversion, which can be beneficial for a more efficient and optimized BES. |
Tipo: | Artigo |
Descrição: | Supplementary material associated with this article can be found, in the online version, at doi: 10.4121/57265539-f6f7-411a-9b2f-f84dbec7cdda. |
URI: | https://hdl.handle.net/1822/85453 |
DOI: | 10.1016/j.watres.2023.120278 |
ISSN: | 0043-1354 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0043135423007145 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_56311_1.pdf Acesso restrito! | 1,42 MB | Adobe PDF | Ver/Abrir |