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https://hdl.handle.net/1822/85285
Título: | Viable but non-culturable state: a strategy for Staphylococcus aureus survivable in dual-species biofilms with Pseudomonas aeruginosa |
Autor(es): | Magalhães, Andreia Patrícia Alves Graínha, Tânia Raquel Rodrigues Sousa, Ana Margarida França, Ângela Maria Oliveira Sousa Cerca, Nuno Pereira, Maria Olívia |
Data: | 5-Set-2019 |
Citação: | Magalhães, Andreia P.; Grainha, Tânia; Sousa, Ana Margarida; França, Angela; Cerca, Nuno; Pereira, Maria Olívia, Viable but non-culturable state: a strategy for Staphylococcus aureus survivable in dual-species biofilms with Pseudomonas aeruginosa. EUROBIOFILMS 2019 - 6th European Congress on Biofilms. No. 164, Glasgow, Scotland, Sep 03-06, 67, 2019. |
Resumo(s): | While considerable research has focused on studying individual-species, we now face the challenge of determining how interspecies interactions alter bacterial behaviours and pathogenesis. Of particular interest is the ecological behaviour between P. aeruginosa-S. aureus, since microbial communities containing both pathogens can display enhanced virulence. Curiously, their interaction is often reported as competitive under laboratory conditions. Here we investigate the interaction between P. aeruginosa-S. aureus in a biofilm co-culture model. The growth of 24- and 48h-old dual-species biofilms was monitored by CFU and flow-cytometry counting, and the gene expression profile inspected by qPCR. Plating experiments showed that S. aureus growth significantly decreased from 24- up to 72-h of growth in dual-species biofilms with P. aeruginosa, suggesting a clear competitive advantage for P. aeruginosa after 24-h. The gene expression profile showed that P. aeruginosa PA14 significantly overexpressed several key virulence-related genes (pqsE, rhlR, pvdE and lasI) after 24-h of growth with S. aureus ATCC 25923, comparatively with singles-pecies biofilms, which could justify the rapid decline of S. aureus population after 24-h of co-culture with P. aeruginosa. Nevertheless, the isolated strain P. aeruginosa 362668 non-mucoid exhibited a reverse pattern by downregulated the expression of those genes during dual-species growth. As P. aeruginosa exoproducts in addition to cause S. aureus lysis or inhibition may also induce biofilm dispersion, the bulk-fluid of 48-h biofilms was analysed by culture counts and flow-cytometry. Interestingly, while no S. aureus cells were detected by CFU, flow-cytometry data revealed the presence of S. aureus in high numbers (~6 Log). The metabolic activity of S. aureus cells was confirmed by measure the expression levels of sodA gene. In conclusion, the presence of S. aureus in a viable but non-cultivable state within dual-species biofilms with P. aeruginosa underscores the impact of interspecies interactions on antibiotic efficacy in the context of polymicrobial infections. |
Tipo: | Comunicação em painel |
URI: | https://hdl.handle.net/1822/85285 |
Versão da editora: | https://www.eurobiofilms2019.com |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_52084_1.pdf | Abstract | 45,34 kB | Adobe PDF | Ver/Abrir |
document_52084_2.pdf | Presentation | 68,99 MB | Adobe PDF | Ver/Abrir |