Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/84847
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Brandão, Ana Catarina | por |
dc.contributor.author | Putzeys, Leena | por |
dc.contributor.author | Pires, Diana Priscila Penso | por |
dc.contributor.author | Voet, Marleen | por |
dc.contributor.author | Paeshuyse, Jan | por |
dc.contributor.author | Azeredo, Joana | por |
dc.contributor.author | Lavigne, Rob | por |
dc.date.accessioned | 2023-06-01T10:57:06Z | - |
dc.date.issued | 2023-05-24 | - |
dc.identifier.citation | Brandão, A. C., Putzeys, L., Pires, D. P., Voet, M., Paeshuyse, J., Azeredo, J., & Lavigne, R. (2023, May 24). Impact of phage predation on P. aeruginosa adhered to human airway epithelium: major transcriptomic changes in metabolism and virulence-associated genes. RNA Biology. Informa UK Limited. http://doi.org/10.1080/15476286.2023.2216065 | por |
dc.identifier.issn | 1547-6286 | por |
dc.identifier.uri | https://hdl.handle.net/1822/84847 | - |
dc.description.abstract | Phage therapy is a promising adjunct therapeutic approach against bacterial multidrug-resistant infections, including Pseudomonas aeruginosa-derived infections. Nevertheless, the current knowledge about the phage-bacteria interaction within a human environment is limited. In this work, we performed a transcriptome analysis of phage-infected P. aeruginosa adhered to a human epithelium (Nuli-1 ATCC® CRL-4011). To this end, we performed RNA-sequencing from a complex mixture comprising phagebacteriahuman cells at early, middle, and late infection and compared it to uninfected adhered bacteria. Overall, we demonstrated that phage genome transcription is unaltered by bacterial growth and phage employs a core strategy of predation through upregulation of prophage-associated genes, a shutdown of bacterial surface receptors, and motility inhibition. In addition, specific responses were captured under lung-simulating conditions, with the expression of genes related to spermidine syntheses, sulphate acquisition, biofilm formation (both alginate and polysaccharide syntheses), lipopolysaccharide (LPS) modification, pyochelin expression, and downregulation of virulence regulators. These responses should be carefully studied in detail to better discern phage-induced changes from bacterial responses against phage. Our results establish the relevance of using complex settings that mimics in vivo conditions to study phage-bacteria interplay, being obvious the phage versatility on bacterial cell invasion. | por |
dc.description.sponsorship | The work was supported by the Fundação para a Ciência e a Tecnologia [SFRH/BD/133193/2017]; Fundação para a Ciência e a Tecnologia [UIDB/04469/2020]; Fundação para a Ciência e a Tecnologia [LA/P/ 0029/2020]; H2020 European Research Council [819800]; H2020 European Research Council [[819800]]; Portuguese Foundation for Science and Technology (FCT) [EXPL/EMD-EMD/1142/2021]; “la Caixa” Foundation [LCF/PR/HP21/52310017]. | por |
dc.language.iso | eng | por |
dc.publisher | Taylor & Francis | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F133193%2F2017/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/819800/EU | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FEMD-EMD%2F1142%2F2021/PT | por |
dc.rights | restrictedAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | P. aeruginosa | por |
dc.subject | bacteriophage | por |
dc.subject | RNA-sequencing | por |
dc.subject | virulence | por |
dc.subject | interactions | por |
dc.title | Impact of phage predation on P. aeruginosa adhered to human airway epithelium: major transcriptomic changes in metabolism and virulence-associated genes | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.tandfonline.com/doi/full/10.1080/15476286.2023.2216065 | por |
dc.comments | CEB56267 | por |
oaire.citationStartPage | 235 | por |
oaire.citationEndPage | 247 | por |
oaire.citationIssue | 1 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationVolume | 20 | por |
dc.date.updated | 2023-06-01T09:49:21Z | - |
dc.identifier.doi | 10.1080/15476286.2023.2216065 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 37226433 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | RNA Biology | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56267_1.pdf Acesso restrito! | 7,58 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons