Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/84377
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Silva, Catarina S. | por |
dc.contributor.author | Kundu, Banani | por |
dc.contributor.author | Gomes, Joana M. | por |
dc.contributor.author | Fernandes, Emanuel Mouta | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Kundu, Subhas C | por |
dc.contributor.author | Martins, Albino | por |
dc.contributor.author | Neves, N. M. | por |
dc.date.accessioned | 2023-05-10T12:33:22Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.citation | Silva C. S., Kundu B., Gomes J. M., Fernandes E. M., Reis R. L., Kundu S. C., Martins A., Neves N. M. Development of bilayered porous silk scaffolds for thymus bioengineering, Biomaterials Advances, Vol. 147, pp. 213320, doi:10.1016/j.bioadv.2023.213320, 2023 | por |
dc.identifier.uri | https://hdl.handle.net/1822/84377 | - |
dc.description.abstract | The thymus coordinates the development and selection of T cells. It is structured into two main compartments: the cortex and the medulla. The replication of such complex 3D environment has been challenged by bioengineering approaches. Nevertheless, the effect of the scaffold microstructure on thymic epithelial cell (TEC) cultures has not been deeply investigated. Here, we developed bilayered porous silk fibroin scaffolds and tested their effect on TEC co-cultures. The small and large pore scaffolds presented a mean pore size of 84.33 ± 21.51 μm and 194.90 ± 61.38 μm, respectively. The highly porous bilayered scaffolds presented a high water absorption and water content (> 94 %), together with mechanical properties in the range of the native tissue. TEC (i.e., medullary (mTEC) and cortical (cTEC) cell lines) proliferation is increased in scaffolds with larger pores. The co-culture of both TEC lines in the bilayered porous silk scaffolds presents enhanced cell proliferation and metabolic activity when compared with mTEC in single culture. Also, when the co-culture occurred with cTEC in the small pores layer and mTEC in the large pores layer, a 9.2- and 18.9-fold increase in Foxn1 and Icam1 gene expression in cTEC is evident. These results suggest that scaffold microstructure and the co-culture influence TEC's behaviour. Bilayered silk scaffolds with adjusted microstructure are a valid alternative for TEC culture, having possible applications in advanced thymus bioengineering strategies. | por |
dc.description.sponsorship | This work was supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, for the PhD grant of C.S.S. (UMINHO/BD/33/2016; NORTE08-5369-FSE-000012) and the HEALTH-UNORTE project (NORTE-01-0145-FEDER-000039). The European Union Framework Programme also supported the work for Research and Innovation Horizon 2020 under grant agreement no 668983 — FoReCaST, where S.C.K. has been the ERA Chair, and Portuguese Foundation for Science and Technology (FCT) project (PTDC/BTM-ORG/28168/2017). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | UMINHO/BD/33/2016 | por |
dc.relation | NORTE08-5369-FSE-000012 | por |
dc.relation | NORTE-01-0145-FEDER-000039 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTM-ORG%2F28168%2F2017/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Bilayered scaffolds | por |
dc.subject | Microarchitecture | por |
dc.subject | Silk fibroin | por |
dc.subject | Thymic epithelial cells | por |
dc.subject | Thymus bioengineering | por |
dc.title | Development of bilayered porous silk scaffolds for thymus bioengineering | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://doi.org/10.1016/j.bioadv.2023.213320 | por |
dc.comments | http://3bs.uminho.pt/node/20936 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 13 | por |
oaire.citationVolume | 147 | por |
dc.date.updated | 2023-05-09T15:29:14Z | - |
dc.identifier.eissn | 2772-9508 | por |
dc.identifier.doi | 10.1016/j.bioadv.2023.213320 | por |
dc.date.embargo | 10000-01-01 | - |
sdum.journal | Biomaterials Advances | por |
dc.identifier.articlenumber | 213320 | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
20936-development-bilayered-porous-silk-scaffolds-thymus-bioengineering.pdf Acesso restrito! | 10,08 MB | Adobe PDF | Ver/Abrir |