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https://hdl.handle.net/1822/84376
Título: | The influence of feeder cell-derived extracellular matrix density on thymic epithelial cell culture |
Autor(es): | Silva, Catarina S. Pinto, Rute D. Pires, R. A. Correia-Neves, M Reis, R. L. Alves, Nuno L. Martins, Albino Neves, N. M. |
Palavras-chave: | Decellularization Electrospun fibrous meshes Extracellular matrix Thymic epithelial cells Thymus bioengineering |
Data: | Abr-2023 |
Editora: | ACS Publications |
Revista: | ACS Biomaterials Science & Engineering |
Citação: | Silva C. S., Pinto R. D., Pires R. P., Correia-Neves M., Reis R. L., Alves N. L., Martins A., Neves N. M. The Influence of Feeder Cell-Derived Extracellular Matrix Density on Thymic Epithelial Cell Culture, Biomaterials Science & Engineering, Vol. 9, pp. 2514 - 2523, doi:10.1021/acsbiomaterials.3c00148, 2023 |
Resumo(s): | The thymus is responsible for the selection and development of T cells, having an essential role in the establishment of adaptive immunity. Thymic epithelial cells (TECs) are key players in T cell development interacting with thymocytes in the thymic 3D environment. Feeder-layer cells have been frequently used as platforms for the successful establishment of TEC cultures. Nevertheless, the role of the feeder cell-derived extracellular matrix (ECM) on TEC cultures was not previously reported. Therefore, this work aimed at assessing the effect of the ECM produced by feeder cells cultured at two different densities on the establishment of TEC culture. Due to the high surface area and porosity, electrospun fibrous meshes were used to support ECM deposition. The feeder cell-derived ECM was efficiently recovered after decellularization, maintaining the composition of major proteins. All the decellularized matrices were permeable and showed an increase in surface mechanical properties after decellularization. TEC cultures confirmed that the ECM density impacts cellular performance, with higher densities showing a decreased cellular activity. Our findings provide evidence that feeder cell-derived ECM is a suitable substrate for TEC culture and can potentially be applied in thymus bioengineering. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/84376 |
DOI: | 10.1021/acsbiomaterials.3c00148 |
ISSN: | 2373-9878 |
e-ISSN: | 2373-9878 |
Versão da editora: | https://pubs.acs.org/doi/10.1021/acsbiomaterials.3c00148 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20935-influence-feeder-cell-derived-extracellular-matrix-density-thymic-epithelial-cell-culture.pdf Acesso restrito! | 7,62 MB | Adobe PDF | Ver/Abrir |