Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/83747
Título: | Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging |
Autor(es): | Vieira, Sílvia Strymecka, Paulina Stanaszek, Luiza Silva-Correia, Joana Drela, Katarzyna Fiedorowicz, Michał Malysz-Cymborska, Izabela Janowski, Miroslaw Reis, R. L. Lukomska, Barbara Walczak, Piotr Oliveira, Joaquim M. |
Palavras-chave: | Cell delivery Gellan Gum Injectable hydrogels Magnetic resonance imaging Manganese |
Data: | Mar-2023 |
Editora: | MDPI |
Revista: | Bioengineering |
Citação: | Vieira S., Strymecka P., Stanaszek L., Silva-Correia J., Drela K., Fiedorowicz M., Malysz-Cymborska I., Janowski M., Reis R. L., Lukomska B., Walczak P., Oliveira J. M. Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging, Bioengineering, Vol. 10, Issue 4, pp. 427, doi:10.3390/bioengineering10040427, 2023 |
Resumo(s): | This work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBPshi/shi/rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/83747 |
DOI: | 10.3390/bioengineering10040427 |
e-ISSN: | 2306-5354 |
Versão da editora: | https://www.mdpi.com/2306-5354/10/4/427 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20921-bioengineering-10-00427-1.pdf | 1,92 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons