Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/82756
Título: | Compilation, revision, and annotation of DNA barcodes of marine invertebrate Non-Indigenous Species (NIS) occurring in european coastal regions |
Autor(es): | Lavrador, Ana Sofia Castro Fontes, João T. Vieira, Pedro Emanuel Ferreira Reis Costa, Filipe O. Duarte, Sofia Alexandra Ferreira |
Palavras-chave: | European coastal ecosystems DNA reference libraries COI barcode sequences |
Data: | 2023 |
Editora: | MDPI |
Revista: | Diversity |
Citação: | Lavrador, A.S.; Fontes, J.T.; Vieira, P.E.; Costa, F.O.; Duarte, S. Compilation, Revision, and Annotation of DNA Barcodes of Marine Invertebrate Non-Indigenous Species (NIS) Occurring in European Coastal Regions. Diversity 2023, 15, 174. https://doi.org/10.3390/d15020174 |
Resumo(s): | The introduction of non-indigenous species (NIS) is one of the major threats to the integrity of European coastal ecosystems. DNA-based assessments have been increasingly adopted for monitoring NIS. However, the accuracy of DNA-based taxonomic assignments is largely dependent on the completion and reliability of DNA barcode reference libraries. As such, we aimed to compile and audit a DNA barcode reference library for marine invertebrate NIS occurring in Europe. To do so, we compiled a list of NIS using three databases: the European Alien Species Information Network (EASIN), the Information System on Aquatic Non-indigenous and Cryptogenic Species (AquaNIS), and the World Register of Introduced Marine Species (WRiMS). For each species, we retrieved the available cytochrome c oxidase subunit I (COI) mitochondrial gene sequences from the Barcode of Life Data System (BOLD) and used the Barcode, Audit & Grade System (BAGS) to check congruence between morphospecies names and Barcode Index Numbers (BINs). From the 1249 species compiled, approximately 42% had records on BOLD, among which 56% were discordant. We further analyzed these cases to determine the causes of the discordances and attributed additional annotation tags. Of the 622 discordant BINs, after revision, 35% were successfully solved, which increased the number of NIS detected in metabarcoding datasets from 12 to 16. However, a fair number of BINs remained discordant. Reliability of reference barcode records is particularly critical in the case of NIS, where erroneous identification may trigger action or inaction when not required. |
Tipo: | Artigo |
Descrição: | The script developed to create the DNA sequence reference library is available at https://github.com/tadeu95/Curated-BINs-Reference-Library (accessed on 25 January 2023). Metabarcoding datasets will be made available upon request since they belong to a study that is not published yet. |
URI: | https://hdl.handle.net/1822/82756 |
DOI: | 10.3390/d15020174 |
e-ISSN: | 1424-2818 |
Versão da editora: | https://www.mdpi.com/1424-2818/15/2/174 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Lavrador et al 2023_auditoria NIS.pdf | 1,89 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons