Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/82637
Título: | Platelet-derived extracellular vesicles promote tenogenic differentiation of stem cells on bioengineered living fibers |
Autor(es): | Graça, Ana L. Domingues, Rui Miguel Andrade Gómez-Florit, Manuel Gomes, Manuela E. |
Palavras-chave: | Extracellular vesicles Platelets Stem cells Tenogenic differentiation Hierarchical scaffolds Composite living fibers |
Data: | Fev-2023 |
Editora: | MDPI |
Revista: | International Journal of Molecular Sciences |
Citação: | Graça A. L., Domingues R. M. A., Gómez-Florit M., Gomes M. E. Platelet-derived extracellular vesicles promote tenogenic differentiation of stem cells on bioengineered living fibers, International journal Of molecular sciences, Vol. 24, Issue 4, pp. 1-18, doi:10.3390/ijms24043516, 2023 |
Resumo(s): | Tendon mimetic scaffolds that recreate the tendon hierarchical structure and niche have increasing potential to fully restore tendon functionality. However, most scaffolds lack biofunctionality to boost the tenogenic differentiation of stem cells. In this study, we assessed the role of platelet-derived extracellular vesicles (EVs) in stem cellsâ tenogenic commitment using a 3D bioengineered in vitro tendon model. First, we relied on fibrous scaffolds coated with collagen hydrogels encapsulating human adipose-derived stem cells (hASCs) to bioengineer our composite living fibers. We found that the hASCs in our fibers showed high elongation and cytoskeleton anisotropic organization, typical of tenocytes. Moreover, acting as biological cues, platelet-derived EVs boosted the hASCsâ tenogenic commitment, prevented phenotypic drift, enhanced the deposition of the tendon-like extracellular matrix, and induced lower collagen matrix contraction. In conclusion, our living fibers provided an in vitro system for tendon tissue engineering, allowing us to study not only the tendon microenvironment but also the influence of biochemical cues on stem cell behavior. More importantly, we showed that platelet-derived EVs are a promising biochemical tool for tissue engineering and regenerative medicine applications that are worthy of further exploration, as paracrine signaling might potentiate tendon repair and regeneration. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/82637 |
DOI: | 10.3390/ijms24043516 |
ISSN: | 1422-0067 |
Versão da editora: | https://www.mdpi.com/1422-0067/24/4/3516 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
20895-ijms-24-03516.pdf | 2,48 MB | Adobe PDF | Ver/Abrir |