Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/82080
Título: | Novel biomimetic Prussian blue nanocubes-based biosensor for Tau-441 protein detection |
Autor(es): | Hassine, Amira Ben Raouafi, Noureddine Moreira, Felismina |
Palavras-chave: | Alzheimer Tau-441, Molecular imprinting polymer Prussian blue nanocubes Biosensor Electrochemical Tau-441 Molecular imprinting polymer |
Data: | 20-Mar-2023 |
Editora: | Elsevier 1 |
Revista: | Journal of Pharmaceutical and Biomedical Analysis |
Citação: | Ben Hassine, A., Raouafi, N., & Moreira, F. T. C. (2023, March). Novel biomimetic Prussian blue nanocubes-based biosensor for Tau-441 protein detection. Journal of Pharmaceutical and Biomedical Analysis. Elsevier BV. http://doi.org/10.1016/j.jpba.2023.115251 |
Resumo(s): | Tau protein is a promising biomarker for early diagnosis of Alzheimer's disease (AD). Therefore, there is an urgent need to develop a simple and effective method for its detection. To this end, an innovative sensing device was developed using a carbon screen-printed electrode (C-SPE) decorated with graphene oxide/Prussian Blue nanocubes (GO/PBNCs) for the selective and sensitive determination of Tau-441 protein. The biorecognition layer was built on the rGO/PBNCs/C-SPE by electropolymerizing 3-aminophenol (3-AMP) in the presence of the target protein using chronoamperometry, and the template was subsequently removed from the polymer matrix with oxalic acid. In parallel, a non-imprinted material (NIP) was also prepared in the absence of the target for comparison purposes. Scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy were used to study the morphology of the modified electrode, and electrochemical techniques were used to monitor the stepwise assembly of the sensor. Under optimized conditions, the sensing platform exhibited a linear range of 1.09 pmol/L and 2.18 nmol/L and a detection limit of 0.01 pmol/L in spiked PBS solution. The molecularly imprinted polymer (MIP) sensor showed minimal interference with uric acid and bovine albumin. The simplicity of production, affordable cost and promising performance make this sensor a potential strategic sensing platform for the detection of chemical and biological molecules. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/82080 |
DOI: | 10.1016/j.jpba.2023.115251 |
ISSN: | 0731-7085 |
Versão da editora: | https://www.sciencedirect.com/science/article/abs/pii/S0731708523000201 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56028_1.pdf Acesso restrito! | 1,55 MB | Adobe PDF | Ver/Abrir |