Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81707
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Dias, Teresa | por |
dc.contributor.author | Santos, Vítor S. | por |
dc.contributor.author | Zorgani, Tarek | por |
dc.contributor.author | Ferreiro, Nuno | por |
dc.contributor.author | Rodrigues, Ana I. | por |
dc.contributor.author | Zaghdoudi, Khalil | por |
dc.contributor.author | Veloso, Ana C. A. | por |
dc.contributor.author | Peres, António M. | por |
dc.date.accessioned | 2023-01-11T10:17:20Z | - |
dc.date.available | 2023-01-11T10:17:20Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Dias, T.; Santos, V.S.; Zorgani, T.; Ferreiro, N.; Rodrigues, A.I.; Zaghdoudi, K.; Veloso, A.C.A.; Peres, A.M. A Lab-Made E-Nose-MOS Device for Assessing the Bacterial Growth in a Solid Culture Medium. Biosensors 2023, 13, 19. https://doi.org/10.3390/bios13010019 | por |
dc.identifier.issn | 2079-6374 | por |
dc.identifier.uri | https://hdl.handle.net/1822/81707 | - |
dc.description.abstract | The detection and level assessment of microorganisms is a practical quality/contamination indicator of food and water samples. Conventional analytical procedures (e.g., culture methods, immunological techniques, and polymerase chain reactions), while accurate and widely used, are time-consuming, costly, and generate a large amount of waste. Electronic noses (E-noses), combined with chemometrics, provide a direct, green, and non-invasive assessment of the volatile fraction without the need for sample pre-treatments. The unique olfactory fingerprint generated during each microorganism’s growth can be a vehicle for its detection using gas sensors. A lab-made E-nose, comprising metal oxide semiconductor sensors was applied, to analyze solid medium containing Gram-positive (Enterococcus faecalis and Staphylococcus aureus) or Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The electrical-resistance signals generated by the E-nose coupled with linear discriminant analysis allowed the discrimination of the four bacteria (90% of correct classifications for leave-one-out cross-validation). Furthermore, multiple linear regression models were also established allowing quantifying the number of colony-forming units (CFU) (0.9428 ≤ R2 ≤ 0.9946), with maximum root mean square errors lower than 4 CFU. Overall, the E-nose showed to be a powerful qualitative–quantitative device for bacteria preliminary analysis, being envisaged its possible application in solid food matrices. | por |
dc.description.sponsorship | This research was funded by the Foundation for Science and Technology (FCT, Portugal), through national funds FCT/MCTES (PIDDAC), to CIMO (UIDB/00690/2020 and UIDP/00690/2020), to CEB (UIDB/04469/2020) and to the Associate Laboratory SusTEC (LA/P/0007/2020). | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | electronic nose | por |
dc.subject | metal oxide semiconductor sensors | por |
dc.subject | bacteria identification | por |
dc.subject | Gram-positive | por |
dc.subject | Gram-negative | por |
dc.subject | colony-forming units | por |
dc.subject | linear discriminant analysis | por |
dc.subject | multiple linear regression models | por |
dc.subject | simulated annealing algorithm | por |
dc.title | A lab-made E-nose-MOS device for assessing the bacterial growth in a solid culture medium | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2079-6374/13/1/19 | por |
dc.comments | CEB56008 | por |
oaire.citationStartPage | 19 | por |
oaire.citationIssue | 1 | por |
oaire.citationConferencePlace | Switzerland | - |
oaire.citationVolume | 13 | por |
dc.date.updated | 2023-01-04T18:26:52Z | - |
dc.identifier.doi | 10.3390/bios13010019 | por |
dc.identifier.pmid | 36671854 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biosensors | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56008_1.pdf | 1,13 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons