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https://hdl.handle.net/1822/81630
Título: | Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx |
Autor(es): | Moreira, Helena R. Rodrigues, Daniel Barreira Ribeiro, Sara Freitas Silva, Lucília Pereira Morais, Alain da S. Jarnalo, Mariana Horta, Ricardo Reis, R. L. Pirraco, Rogério P. Marques, A. P. |
Palavras-chave: | Inflammation Integrin-specific biomaterials Re-epithelization Remodeling Stromal vascular fraction Wound healing |
Data: | Dez-2022 |
Editora: | Elsevier 1 |
Revista: | Materials Today Bio |
Citação: | Moreira H. R., Rodrigues D. B., Freitas-Ribeiro S., da Silva L. P., Morais A., Jarnalo M., Horta R., Reis R. L., Pirraco R. P., Marques A. P. Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx, Materials Today Bio, Vol. 17, pp. 100496, doi:10.1016/j.mtbio.2022.100496, 2022 |
Resumo(s): | In vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important in cutaneous wound healing. Thus, we aimed to investigate whether in vitro construct prevascularization with SVF accelerates the healing cascade by fostering early vascularization vis-Ã -vis SVF seeding prior to implantation. Prevascularized constructs delayed re-epithelization of full-thickness mice wounds compared to both non-prevascularized and control (no SVF) groups. Our results suggest this delay is due to a persistent inflammation as indicated by a significantly lower M2(CD163+)/M1(CD86+) macrophage subtype ratio. Moreover, a slower transition from the inflammatory to the proliferative phase of the healing was confirmed by reduced extracellular matrix deposition and increased presence of thick collagen fibers from early time-points, suggesting the prevalence of fiber crosslinking in relation to neodeposition. Overall, while prevascularization potentiates inflammatory cell influx, which negatively impacts the cutaneous wound healing cascade, an effective wound healing was guaranteed in non-prevascularized SVF cell-containing spongy-like hydrogels confirming that the SVF can have enhanced efficacy. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/81630 |
DOI: | 10.1016/j.mtbio.2022.100496 |
ISSN: | 2590-0064 |
e-ISSN: | 2590-0064 |
Versão da editora: | https://doi.org/10.1016/j.mtbio.2022.100496 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20876-1-s20-s2590006422002940-main.pdf | 4,66 MB | Adobe PDF | Ver/Abrir |