Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81629
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Moreira, Helena R. | por |
dc.contributor.author | Rodrigues, Daniel Barreira | por |
dc.contributor.author | Ribeiro, Sara Freitas | por |
dc.contributor.author | Silva, Lucília Pereira | por |
dc.contributor.author | Morais, Alain da S. | por |
dc.contributor.author | Jarnalo, Mariana | por |
dc.contributor.author | Horta, Ricardo | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Pirraco, Rogério P. | por |
dc.contributor.author | Marques, A. P. | por |
dc.date.accessioned | 2023-01-09T12:07:34Z | - |
dc.date.available | 2023-01-09T12:07:34Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.citation | Moreira H. R., Rodrigues D. B., Freitas-Ribeiro S., da Silva L. P., Morais A., Jalano M., Horta R., Reis R. L., Pirraco R. P., Marques A. P. Integrin-specific hydrogels for growth factor-free vasculogenesis, npj Regenerative Medicine, Vol. 7, pp. 57, doi:10.1038/s41536-022-00253-4, 2022 | por |
dc.identifier.uri | https://hdl.handle.net/1822/81629 | - |
dc.description.abstract | Integrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascular fraction (SVF) of adipose tissue, triggering vasculogenesis in vitro in the absence of extrinsic growth factors. Our results suggest that αvβ3-RGD-driven signaling in the formation of capillary-like structures prevents the activation of the caspase 8 pathway and activates the FAK/paxillin pathway, both responsible for endothelial cells (ECs) survival and migration. We also show that prevascularized αvβ3 integrin-specific constructs inosculate with the host vascular system fostering in vivo neovascularization. Overall, this work demonstrates the ability of the biomaterial to trigger vasculogenesis in an integrin-specific manner, by activating essential pathways for EC survival and migration within a self-regulatory growth factor microenvironment. This strategy represents an improvement to current vascularization routes for Tissue Engineering constructs, potentially enhancing their clinical applicability. | por |
dc.description.sponsorship | The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016-COG-726061) and the Starting Grant “CapBed” (ERC2018-STG-805411), to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08- 5369-FSE-000037 (H.R.M.), and to FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) through the grants SFRH/BD/119756/2016 (D.B.R.), Ph.D. grant PD/BD/135252/2017 (S.F.R.) and IF/00347/ 2015 (R.P.P.). | por |
dc.language.iso | eng | por |
dc.publisher | Springer Nature | por |
dc.relation | ERC-2016-COG-726061 | por |
dc.relation | ERC2018-STG-805411 | por |
dc.relation | PD/169/2013 | por |
dc.relation | NORTE-08-5369-FSE-000037 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F119756%2F2016/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F135252%2F2017/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00347%2F2015%2FCP1294%2FCT0004/PT | por |
dc.rights | openAccess | por |
dc.subject | Biomaterials | por |
dc.subject | Stromal vascular fraction | por |
dc.subject | Vascularization | por |
dc.subject | Vasculogenesis | por |
dc.title | Integrin-specific hydrogels for growth factor-free vasculogenesis | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.nature.com/articles/s41536-022-00253-4 | por |
dc.comments | http://3bs.uminho.pt/node/20877 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 13 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 7 | por |
dc.date.updated | 2023-01-03T11:11:13Z | - |
dc.identifier.eissn | 2057-3995 | por |
dc.identifier.doi | 10.1038/s41536-022-00253-4 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | npj Regenerative Medicine | por |
dc.identifier.articlenumber | 57 | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20877-s41536-022-00253-4-1.pdf | 3,58 MB | Adobe PDF | Ver/Abrir |