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https://hdl.handle.net/1822/8158
Título: | Immobilization of b-galactosidase from kluyveromyces lactis onto a polysiloxane–polyvinyl alcohol magnetic (mPOS–PVA) composite for lactose hydrolysis |
Autor(es): | Neri, D. F. M. Balcão, V. M. Carneiro-da-Cunha, Maria G. Carvalho Junior, L. B. Teixeira, J. A. |
Palavras-chave: | b-Galactosidase Kluyveromyces lactis Covalent immobilization Polysiloxane Polyvinyl alcohol Magnetic support β-Galactosidase |
Data: | Ago-2008 |
Editora: | Elsevier B.V. |
Revista: | Catalysis Communications |
Citação: | "Catalysis Communications". ISSN 1566-7367. 9:14 (Aug. 2008) 2334-2339. |
Resumo(s): | β-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS–PVA, using glutaraldehyde as activating agent and its properties were evaluated. The enzymatic water insoluble derivative displayed the same optimum pH (6.5) and optimum temperature (50 °C) of the soluble enzyme. The apparent Km app and activation energy for both soluble and immobilized enzyme derivative were found to be not significantly different. The mPOS–PVA β-galactosidase preparation presented a higher operational and thermal stability than the soluble enzyme. This immobilized β-galactosidase also was effective in hydrolyzing lactose from milk. Hence, one can conclude that mPOS–PVA is an attractive and efficient support for β-galactosidase immobilization. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/8158 |
DOI: | 10.1016/j.catcom.2008.05.022 |
ISSN: | 1566-7367 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Neri_Catalysis_Comm[1].pdf | 512,16 kB | Adobe PDF | Ver/Abrir |