Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81264
Título: | Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays |
Autor(es): | Abrantes, Mafalda Rodrigues, Diana Domingues, Telma Nemala, Siva S Monteiro, Patricia Borme, Jérôme Alpuim, P. Jacinto, Luis |
Palavras-chave: | Animals Mice Dopamine Graphite Biosensing Techniques Aptamers Nucleotide Brain Diseases Graphene Field-effect transistor Aptasensor LOD Parkinson's disease |
Data: | 24-Nov-2022 |
Editora: | Springer Nature |
Revista: | Journal of Nanobiotechnology |
Citação: | Abrantes, M., Rodrigues, D., Domingues, T. et al. Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays. J Nanobiotechnol 20, 495 (2022). https://doi.org/10.1186/s12951-022-01695-0 |
Resumo(s): | Detecting physiological levels of neurotransmitters in biological samples can advance our understanding of brain disorders and lead to improved diagnostics and therapeutics. However, neurotransmitter sensors for real-world applications must reliably detect low concentrations of target analytes from small volume working samples. Herein, a platform for robust and ultrasensitive detection of dopamine, an essential neurotransmitter that underlies several brain disorders, based on graphene multitransistor arrays (gMTAs) functionalized with a selective DNA aptamer is presented. High-yield scalable methodologies optimized at the wafer level were employed to integrate multiple graphene transistors on small-size chips (4.5 × 4.5 mm). The multiple sensor array configuration permits independent and simultaneous replicate measurements of the same sample that produce robust average data, reducing sources of measurement variability. This procedure allowed sensitive and reproducible dopamine detection in ultra-low concentrations from small volume samples across physiological buffers and high ionic strength complex biological samples. The obtained limit-of-detection was 1 aM (10-18) with dynamic detection ranges spanning 10 orders of magnitude up to 100 µM (10-8), and a 22 mV/decade peak sensitivity in artificial cerebral spinal fluid. Dopamine detection in dopamine-depleted brain homogenates spiked with dopamine was also possible with a LOD of 1 aM, overcoming sensitivity losses typically observed in ion-sensitive sensors in complex biological samples. Furthermore, we show that our gMTAs platform can detect minimal changes in dopamine concentrations in small working volume samples (2 µL) of cerebral spinal fluid samples obtained from a mouse model of Parkinson's Disease. The platform presented in this work can lead the way to graphene-based neurotransmitter sensors suitable for real-world academic and pre-clinical pharmaceutical research as well as clinical diagnosis. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/81264 |
DOI: | 10.1186/s12951-022-01695-0 |
ISSN: | 2158-7027 |
e-ISSN: | 2158-7043 |
Versão da editora: | https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01695-0 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals CDF - CEP - Artigos/Papers (with refereeing) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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J. Nanobiotechnology 20 (2022) 495.pdf | Open access article | 2,47 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons