Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/80634
Título: | Assessing recovery rates of distinct exogenous controls for gDNA extraction efficiency using phenol-chloroform or silica-column based extractions |
Autor(es): | Lima, Ângela Martins Sousa, Lúcia Filipa Guimarães Vieira Macedo, Filipa Muzny, Christina A. Cerca, Nuno |
Palavras-chave: | gDNA extraction efficiency gDNA qPCR bacterial quantification Exogenous controls |
Data: | Dez-2022 |
Editora: | Elsevier 1 |
Revista: | Journal of Microbiological Methods |
Citação: | Lima, Â.; Sousa, Lúcia; Macedo, Filipa; Muzny, Christina A.; Cerca, Nuno, Assessing recovery rates of distinct exogenous controls for gDNA extraction efficiency using phenol-chloroform or silica-column based extractions. Journal of Microbiological Methods, 203(106607), 2022 |
Resumo(s): | Assessment of genomic DNA (gDNA) extraction efficiency is required for accurate bacterial quantification by qPCR. Exogenous DNA molecules are often added after bacterial cultures are lysed, but before DNA purification steps, to determine extraction efficiency. Herein we found that different exogenous DNA controls have different recovery rates, suggesting distinct DNA extraction efficiencies. Recovery rates are also affected by the gDNA extraction method being more affected in silica-based columns than in phenol-chloroform extraction. Overall, we determined that the use of long DNA fragments, such as gDNA, as exogenous controls have a higher recovery rate than use of smaller size DNA molecules. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/80634 |
DOI: | 10.1016/j.mimet.2022.106607 |
ISSN: | 01677012 |
Versão da editora: | http://www.journals.elsevier.com/journal-of-microbiological-methods/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55866_1.pdf | 1,21 MB | Adobe PDF | Ver/Abrir |