Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/80471
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Sande, Maria Georgina | por |
dc.contributor.author | Roque, Lúcia | por |
dc.contributor.author | Braga, Adelaide Correia | por |
dc.contributor.author | Marques, Márcia | por |
dc.contributor.author | Ferreira, Débora Carina Gonçalves Abreu | por |
dc.contributor.author | Saragliadis, Athanasios | por |
dc.contributor.author | Rodrigues, Joana Lúcia Lima Correia | por |
dc.contributor.author | Linke, Dirk | por |
dc.contributor.author | Ramada, David | por |
dc.contributor.author | Silva, C. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2022-11-07T15:03:49Z | - |
dc.date.issued | 2023-02 | - |
dc.identifier.citation | Sande, MG, Roque, L, Braga, A, et al. Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pathogens. J Biomed Mater Res. 2023; 111( 2): 354- 365. doi:10.1002/jbm.b.35155 | por |
dc.identifier.issn | 1552-4973 | por |
dc.identifier.uri | https://hdl.handle.net/1822/80471 | - |
dc.description.abstract | Enrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time needed to confirm the presence of a bacterial infection. MNPs were synthesized by a simple two-step approach through a green co-precipitation method using water as solvent. These MNPs were specifically designed to interact with pathogenic bacteria by establishing a hydrolyzed collagen-adhesin linker. The bacterial capture efficacy of hydrolyzed collagen MNPs (H-Coll | eng |
dc.description.sponsorship | The study received financial support from ViBrANT project that received funding from the EU Horizon 2020 Research and Innovation Programme under the Marie Sklowdowska-Curie, Grant agreement no. 765042 and Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. DL and AS receive additional funding from the Research Council of Norway (grant no. 294605, Center for Digital Life). The authors acknowledge Diana Vilas Boas from Centre of Biological Engineering for technical assistance in confocal scanning laser microscopy. The TEM experiments were carried out at the INL Advanced Electron Microscopy, Imaging and Spectroscopy Facility. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/765042/EU | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Adhesins | por |
dc.subject | Arginine magnetic nanoparticles | por |
dc.subject | Bacterial adhesion | por |
dc.subject | Enrichment | por |
dc.subject | Hydrolyzed collagen magnetic nanoparticles | por |
dc.subject | Ubiquitous surface protein A2 (UspA2) | por |
dc.subject | Yersinia adhesin A (YadA) | por |
dc.title | Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pathogens | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35155 | por |
dc.comments | CEB55749 | por |
oaire.citationStartPage | 354 | - |
oaire.citationEndPage | 365 | - |
oaire.citationIssue | 2 | - |
oaire.citationConferencePlace | United States | - |
oaire.citationVolume | 111 | - |
dc.date.updated | 2022-11-05T09:47:13Z | - |
dc.identifier.eissn | 1552-4981 | por |
dc.identifier.doi | 10.1002/jbm.b.35155 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 36063491 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Biomedical Materials Research - Part B: Applied Biomaterials | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_55749_1.pdf Acesso restrito! | 4,19 MB | Adobe PDF | Ver/Abrir |