Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/80254
Título: | A new fluorogenic substrate for granzyme B based on fluorescence resonance energy transfer |
Autor(es): | Martins, Cátia D. F. Raposo, M. Manuela M. Costa, Susana P. G. |
Palavras-chave: | donor–acceptor pair FRET granzyme B fluorescent probes peptides synthesis |
Data: | 2021 |
Editora: | MDPI |
Revista: | Chemistry Proceedings |
Citação: | Martins, C.D.F.; Raposo, M.M.M.; Costa, S.P.G. A New Fluorogenic Substrate for Granzyme B Based on Fluorescence Resonance Energy Transfer. Chem. Proc. 2021, 3, 6. https://doi.org/10.3390/ecsoc-24-08311 |
Resumo(s): | The synthesis and characterization of a new fluorogenic substrate for granzyme B (GzmB) is reported. The substrate design was based on the fluorescence resonance energy transfer (FRET) principle using 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (Edans) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) as a donor–acceptor pair, linked to a specific sequence for GzmB (AAD), with an additional amino acid as the anchoring point (K). The tetrapeptide was synthesized by microwave-assisted solid-phase peptide synthesis (MW-SPPS) and coupled to Dabcyl and Edans at its N- and C-termini, respectively. The obtained probe was purified by semi-preparative HPLC and characterized by NMR, UV/Vis absorption and fluorescence spectroscopy and mass spectrometry. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/80254 |
DOI: | 10.3390/ecsoc-24-08311 |
e-ISSN: | 2673-4583 |
Versão da editora: | https://www.mdpi.com/2673-4583/3/1/6 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDQuim - Comunicações e Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Chem. Proc. 2021, 3, 6.pdf | artigo em livro de atas | 411,15 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons