Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/79901
Título: | Methodology for phytoplankton taxonomic group identification towards the development of a lab-on-a-chip |
Autor(es): | Carvalho, Denise Andreia Mota Pinto, Vânia Cristina Gonçalves Sousa, Paulo Jorge Teixeira Magalhães, Vitor H. Fernández, Emilio Gomes, Pedro A. Minas, Graça Gonçalves, Luís M. |
Palavras-chave: | phytoplankton sensor optical methods photosynthetic pigment lab-on-a-chip |
Data: | 26-Mai-2022 |
Editora: | Multidisciplinary Digital Publishing Institute |
Revista: | Applied Sciences |
Citação: | Carvalho, D.A.M.; Pinto, V.C.; Sousa, P.J.; Magalhães, V.H.; Fernández, E.; Gomes, P.A.; Minas, G.; Gonçalves, L.M. Methodology for Phytoplankton Taxonomic Group Identification towards the Development of a Lab-on-a-Chip. Appl. Sci. 2022, 12, 5376. https://doi.org/10.3390/app12115376 |
Resumo(s): | This paper presents the absorbance and fluorescence optical properties of various phytoplankton species, looking to achieve an accurate method to detect and identify a number of phytoplankton taxonomic groups. The methodology to select the excitation and detection wavelengths that results in superior identification of phytoplankton is reported. The macroscopic analyses and the implemented methodology are the base for designing a lab-on-a-chip device for a phytoplankton group identification, based on cell analysis with multi-wavelength lighting excitation, aiming for a cheap and portable platform. With such methodology in a lab-on-a-chip device, the analysis of the phytoplankton cells’ optical properties, e.g., fluorescence, diffraction, absorption and reflection, will be possible. This device will offer, in the future, a platform for continuous, autonomous and in situ underwater measurements, in opposition to the conventional methodology. A proof-of-concept device with LED light excitation at 450 nm and a detection photodiode at 680 nm was fabricated. This device was able to quantify the concentration of the phytoplankton chlorophyll a. A lock-in amplifier electronic circuit was developed and integrated in a portable and low-cost sensor, featuring continuous, autonomous and in situ underwater measurements. This device has a detection limit of 0.01 µ/L of chlorophyll a, in a range up to 300 µg/L, with a linear voltage output with chlorophyll concentration. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/79901 |
DOI: | 10.3390/app12115376 |
e-ISSN: | 2076-3417 |
Versão da editora: | https://www.mdpi.com/2076-3417/12/11/5376 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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applsci-12-05376.pdf | 7,93 MB | Adobe PDF | Ver/Abrir |