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https://hdl.handle.net/1822/79773
Título: | The acinetobacter baumannii SOS gene ddrR is crucial for prophage maintenance and induction |
Autor(es): | Monteiro, Rodrigo Pimenta Oliveira Melo, Luís Daniel Rodrigues Domingues, Rita Oliveira, Hugo Alexandre Mendes van Dijl, Jan Maarten Azeredo, Joana |
Palavras-chave: | Prophage SOS-response Acinetobacter baumannii |
Data: | 17-Jun-2022 |
Citação: | Monteiro, Rodrigo; Melo, Luís Daniel Rodrigues; Domingues, Rita; Oliveira, Hugo; van Dijl, Jan Maarten; Azeredo, Joana, The Acinetobacter baumannii SOS gene ddrR is crucial for prophage maintenance and induction. VoM 2022 - Viruses of Microbes - The Latest Conquests (Program and Abstract Book). Guimarães, Portugal, June 17-22, 380, 2022. |
Resumo(s): | Acinetobacter baumannii, considered a number one priority pathogen by WHO, is threatening hospitals due to its high ability to acquire antibiotic resistance. We have recently shown that mobile elements, such as prophages, are highly prevalent and encode several fitness/virulence-related genes, suggesting that they may serve as vectors for the spread of virulence. Here, we want to understand how stress factors influence the prophage behavior and disclose the role of the unique SOS response system, umuDAb and ddrR, in prophage induction. First, the A. baumannii ATCC 17978 strain was subjected to different sub-mic concentrations of mitomycin C (MMC), H O , and ciprofloxacin (cip), followed by incubation, RNA extraction and qRT-PCR analysis. After verifying the prophage behavior under these stress conditions, a ddrR knockout mutant was engineered using CRISPR-Cas9 to assess its influence on prophage expression. As expected, all conditions triggered an SOS response in the type strain as well as prophage induction, as both the ddrR and umuDAb genes, and the cro and capsid genes were overexpressed. Different levels of induction were observed between the conditions tested. For example, prophage induction was lower when challenged with cip than with MMC. In terms of growth curves, we observed that the ddrR mutant grew at faster than the type strain. Curiously, when challenged with MMC, the type strain showed significantly decreased CFUs in contrast to the ddrR mutant strain. Our findings show that each stress condition leads to different levels of prophage responses and that some can increase the fitness/virulence expression without detrimental effects on the host. The SOS gene ddrR is important for prophage induction and, consequently, its absence contributes to the bacterial robustness during growth. In conclusion, it is important to understand how prophages are affected by host genes under different stresses to improve antimicrobial efficacy. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/79773 |
Versão da editora: | https://www.vom2022.org/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55776_1.pdf | 128,41 kB | Adobe PDF | Ver/Abrir |