Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/79745
Título: | Lactoferrin perturbs intracellular trafficking, disrupts cholesterol-rich lipid rafts and inhibits glycolysis of highly metastatic cancer cells harbouring plasmalemmal V-ATPase |
Autor(es): | Pereira, Cátia Sofia Santos Guedes, Joana Catarina Pereira Ferreira, Débora Carina Gonçalves Abreu Rodrigues, L. R. Côrte-Real, Manuela |
Palavras-chave: | Lactoferrin Lipid rafts V-ATPase |
Data: | Nov-2022 |
Editora: | Elsevier 1 |
Revista: | International Journal of Biological Macromolecules |
Citação: | Cátia S. Pereira; Guedes, Joana P.; Ferreira, Débora; Rodrigues, Lígia R.; Côrte-Real, Manuela, Lactoferrin perturbs intracellular trafficking, disrupts cholesterol-rich lipid rafts and inhibits glycolysis of highly metastatic cancer cells harbouring plasmalemmal V-ATPase. International Journal of Biological Macromolecules, 220, 1589-1604, 2022 |
Resumo(s): | The milk-derived bovine lactoferrin (bLf) is an iron-binding glycoprotein with remarkable selective anticancer activity towards highly metastatic cancer cells displaying the proton pump V-ATPase at the plasma membrane. As studies aiming to dissect the bLf mechanisms of action are critical to improve its efficacy and boost its targeted clinical use, herein we sought to further uncover the molecular basis of bLf anticancer activity. We showed that bLf co-localizes with V-ATPase and cholesterol-rich lipid rafts at the plasma membrane of highly metastatic cancer cells. Our data also revealed that bLf perturbs cellular trafficking, induces intracellular accumulation of cholesterol and lipid rafts disruption, downregulates PI3K and AKT or p-AKT and inhibits glycolysis of cancer cells harbouring V-ATPase at the plasma membrane lipid rafts. Altogether, our results can lay the foundation for future bLf-based targeted anticancer strategies as they unravel a novel cascade of molecular events that explains and further reinforces bLf selectivity for cancer cells displaying plasmalemmal V-ATPase. |
Tipo: | Artigo |
Descrição: | Available online 16 September 2022 |
URI: | https://hdl.handle.net/1822/79745 |
DOI: | 10.1016/j.ijbiomac.2022.09.120 |
ISSN: | 0141-8130 |
Versão da editora: | http://www.elsevier.com/locate/issn/01418130 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55744_1.pdf Acesso restrito! | 22,36 MB | Adobe PDF | Ver/Abrir |