Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/79146
Título: | Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
Autor(es): | Mota, Renato Rodrigues, Ana Cristina Silva-Carvalho, Ricardo Costa, Lígia Martins, Daniela Sampaio, Paula Dourado, Fernando Gama, F. M. |
Palavras-chave: | bacterial nanocellulose bacterial cellulose nanocrystals cellulose binding module fluorescence microscopy gastrointestinal tract absorption food additive |
Data: | 28-Jul-2022 |
Editora: | MDPI |
Revista: | Nanomaterials |
Citação: | Mota, Renato; Rodrigues, Ana Cristina; Silva-Carvalho, Ricardo; Costa, Lígia; Martins, Daniela; Sampaio, Paula; Dourado, Fernando; Gama, F. M., Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy. Nanomaterials, 12(15), 2605, 2022 |
Resumo(s): | The potential of nanomaterials in food technology is nowadays well-established. However, their commercial use requires a careful risk assessment, in particular concerning the fate of nanomaterials in the human body. Bacterial nanocellulose (BNC), a nanofibrillar polysaccharide, has been used as a food product for many years in Asia. However, given its nano-character, several toxicological studies must be performed, according to the European Food Safety Agency’s guidance. Those should especially answer the question of whether nanoparticulate cellulose is absorbed in the gastrointestinal tract. This raises the need to develop a screening technique capable of detecting isolated nanosized particles in biological tissues. Herein, the potential of a cellulose-binding module fused to a green fluorescent protein (GFP–CBM) to detect single bacterial cellulose nanocrystals (BCNC) obtained by acid hydrolysis was assessed. Adsorption studies were performed to characterize the interaction of GFP–CBM with BNC and BCNC. Correlative electron light microscopy was used to demonstrate that isolated BCNC may be detected by fluorescence microscopy. The uptake of BCNC by macrophages was also assessed. Finally, an exploratory 21-day repeated-dose study was performed, wherein Wistar rats were fed daily with BNC. The presence of BNC or BCNC throughout the GIT was observed only in the intestinal lumen, suggesting that cellulose particles were not absorbed. While a more comprehensive toxicological study is necessary, these results strengthen the idea that BNC can be considered a safe food additive. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/79146 |
DOI: | 10.3390/nano12152605 |
ISSN: | 2079-4991 |
Versão da editora: | https://www.mdpi.com/2079-4991/12/15/2605 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55664_1.pdf | 2,73 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons