Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/79106
Título: | Engineering Saccharomyces cerevisiae for the one-step production of a functional sweetening mixture towards food applications |
Autor(es): | Amorim, Cláudia Rodrigues, Joana Lúcia Lima Correia Braga, Adelaide Gomes, Daniela Filipa Correia Costa, João Manuel Rainha Silvério, Sara Isabel Cruz Cardoso, Beatriz Fernández-Lobato, María Rodrigues, L. R. |
Palavras-chave: | Fructooligosaccharides Prebiotics Bioprocess Co-culture Recombinant ß-fructofuranosidase Saccharomyces cerevisiae Recombinant β-fructofuranosidase |
Data: | Set-2022 |
Editora: | Institution of Chemical Engineers (IChemE) |
Revista: | Food and Bioproducts Processing |
Citação: | Amorim, Cláudia; Rodrigues, Joana L.; Braga, Adelaide; Gomes, Daniela; Rainha, João; Silvério, Sara C.; Cardoso, B.; Fernández-Lobato, María; Rodrigues, Lígia R., Engineering Saccharomyces cerevisiae for the one-step production of a functional sweetening mixture towards food applications. Food and Bioproducts Processing, 135, 123-134, 2022 |
Resumo(s): | Fructooligosaccharides (FOS) are recognized prebiotics with proven beneficial effects, usually produced by two-step processes. In this work, an in vivo bioprocess was developed for the one-step production of a functional sweetening mixture containing FOS. Sucrose was directly fermented by yeast towards process simplification and cost reduction. Saccharomyces cerevisiae YIL162W lacking the gene responsible for sucrose hydrolysis (SUC2) was transformed to express -fructofuranosidase FTase inv gene from Schwanniomyces occidentalis (clone GAL1_S196), and its mutated version (clone GAL1_L196), under the inducible galactokinase (GAL1) promotor. The FTase inv mutated gene was also expressed under the glyceraldehyde-3-phosphate dehydrogenase (GPD) constitutive promoter (clone GPD_L196). GPD_L196 clone showed the highest potential for FOS production, achieving a maximum productivity of 8.9±0.4mg.g-1.h-1 after 60h, with sucrose supplementation by two pulses, 150g.L-1 at 0 and 24h. When co-cultured with S. cerevisiae wild type (WT), GPD_L196 clone achieved the maximum productivity of 10.9±0.5mg.g-1.h-1 (48h) with an 1:200 inoculum ratio (WT:GPD_L196) and 150g.L-1 of sucrose added at 0 and 24h. After partial purification, a sweetening mixture composed by 75±2% of FOS and 24±2% of sucrose was obtained. This bioprocess approach presents high potential for industrial production of functional sweetening mixtures towards food applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/79106 |
DOI: | 10.1016/j.fbp.2022.07.006 |
ISSN: | 0960-3085 |
Versão da editora: | http://www.elsevier.com/locate/issn/09603085 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55626_1.pdf Acesso restrito! | 1,23 MB | Adobe PDF | Ver/Abrir |