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https://hdl.handle.net/1822/78822
Título: | Study of different pretreatments on Spirulina platensis biomass for bioethanol production |
Autor(es): | Velazquez-Lucio, Jesus Colla, Luciane M. Fernandes, Bruno D. Rodriíguez-Jasso, Rosa M. Ruiz, Héctor A. |
Data: | 28-Jul-2019 |
Citação: | Velazquez-Lucio, Jesus; Colla, Luciane M.; Fernandes, Bruno D.; Rodriíguez-Jasso, Rosa M.; Ruiz, Héctor A., Study of different pretreatments on Spirulina platensis biomass for bioethanol production. SINAFERM 2019 XXII Simpósio Nacional de Bioprocessos. Uberlândia MG, Brazil, July 29-31, 2019. |
Resumo(s): | Aquatic biomass presents a large variety of compounds that can be used for the production of third generation (3G) biofuels, mainly carbohydrates, lipids, proteins and co-products, which can be obtained and used in the production of biofuels such as bioethanol from rich carbohydrate biomass [1]. Nowadays Spirulina platensis biomass can be considered as an alternative since it has a great capacity to produce carbohydrates [2]. This work presents a study of 3G biorefinery process from Spirulina platensis biomass; diverse types of hydrothermal pretreatments (autoclave 121 °C 20 min; freezing/thawing -4 °C and gelatinization 100 °C 10 min; gelatinization 100 °C 20 min; microwave 121 °C 20 min; ultrasound bath 20 min) and their effects on enzymatic hydrolysis with -amylase and amyloglucosidase in order to obtain fermentable sugars were evaluated. Moreover, two fermentation strategies were evaluated; simultaneous saccharification and fermentation (SSF) and pre-saccharification and fermentation (PSF), the conditions used for the fermentation were pH 4.5, 35 ° C, 150 rpm and Saccharomyces cerevisiae yeast was employed, all strategies were used as alternatives in 3G bioethanol process. Results showed that the pretreatment with autoclave (121 ° C 20 min 5% solids) was better for the cellular breakdown and accessibility of enzymes to cellular matrix in the enzymatic hydrolysis. The treatment of pre-saccharification and fermentation (PSF) with 5 % solids pretreated with autoclave at 121 ° C for 20 min and pre-hydrolyzed with -amylase and amyloglucosidase after fermentation obtained a maximum yield of conversion of glucose to bioethanol of 79.34 %. Simultaneous saccharification and fermentation (SSF) was the best strategy for the obtention of bioethanol from pretreatment biomass of Spirulina platensis with a yield of 81.12 %. These results are good since there are no previously reported studies of the use of SSF for bioethanol from microalgae biomass production. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/78822 |
Versão da editora: | https://proceedings.science/sinaferm-sheb-2019/home |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55627_1.pdf | 715,9 kB | Adobe PDF | Ver/Abrir |