Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78691
Título: | Wine astringent compounds monitored by an electrochemical biosensor |
Autor(es): | Costa, Joana J. Moreira, Felismina Soares, Susana Brandão, Elsa Mateus, Nuno Freitas, Victor De Sales, M. G. F. |
Palavras-chave: | Polyphenols Proline Rich Protein a-amylase Screen-printed electrodes astringent compounds electrochemical biosensor ?-Amylase |
Data: | 30-Nov-2022 |
Editora: | Elsevier 1 |
Revista: | Food Chemistry |
Citação: | Costa, Joana J.; Moreira, Felismina; Soares, Susana; Brandão, Elsa; Mateus, Nuno; Freitas, Victor De; Sales, M. G. F., Wine astringent compounds monitored by an electrochemical biosensor. Food Chemistry, 395(133587), 2022. |
Resumo(s): | An innovative approach for monitoring astringent polyphenols in beverages (wines) is described, consisting of an electrochemical biosensor constructed by adsorbing salivary -amylase or proline-rich protein (PRP) onto amined gold screen-printed electrodes. Interaction with polyphenols was tested using pentagalloyl glucose (PGG) as a standard, an important representative element for astringency. The analytical properties of the resulting biosensors were evaluated by electrochemical impedance spectroscopy at different pHs. The PRP-biosensor was able to bind to PGG with higher sensitivity, displaying lower limit of the linear range of 0.6 µM. Wine samples were tested to prove the concept and the concentrations obtained ranged from 0.17 to 4.7 µM, as expressed in PGG units. The effects of side-compounds on PRP and on -amylase binding to PGG were tested (gallic acid, catechin, ethanol, glucose, fructose and glycerol) and considered negligible. Overall, concentrations >1.0 µM in PGG units are signaling electrochemical impedance, providing a quantitative monitoring of astringent compounds. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/78691 |
DOI: | 10.1016/j.foodchem.2022.133587 |
ISSN: | 0308-8146 |
Versão da editora: | https://www.sciencedirect.com/science/article/abs/pii/S0308814622015497 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55615_1.pdf Acesso restrito! | 1,34 MB | Adobe PDF | Ver/Abrir |