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https://hdl.handle.net/1822/78596
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Campo DC | Valor | Idioma |
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dc.contributor.author | Costa, Carlos E. | por |
dc.contributor.author | Carvalho, Patrícia | por |
dc.contributor.author | Domingues, Lucília | por |
dc.date.accessioned | 2022-07-04T10:00:37Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.citation | Costa, Carlos E.; Carvalho, Patrícia; Domingues, Lucília, Strategic combination of different promoters in lactose metabolisation and host chassis selection for high bioethanol titres from dairy wastes. Bioresource Technology Reports, 19(101131), 2022 | por |
dc.identifier.uri | https://hdl.handle.net/1822/78596 | - |
dc.description | "Available online 22 June 2022" | por |
dc.description.abstract | The yeast Saccharomyces cerevisiae is widely used in biotechnological processes but is unable to metabolise lactose, the main component of cheese whey. Here, we engineered industrial S. cerevisiae strains for bioethanol fermentation from lactose. Heterologous expression of LAC12 and LAC4 from Kluyveromyces lactis, encoding for a lactose permease and a -galactosidase, respectively, under different promoter combinations (TEF1p/PGK1p), generated the strains E1 (TEF1pLAC4/PGK1pLAC12) and E2 (PGK1pLAC4/TEF1pLAC12). In synthetic media, E1 exhibits faster lactose consumption up to 150g/L of lactose, while E2 exhibits improved lactose metabolisation ability at 200g/L of lactose with a yield near 100%. These results were validated in whey where the strain E2 produced 92.2g/L of ethanol from 200g/L lactose, the highest titre reported in the literature from whey. This study provides valuable insight on how the promoter choice influences multi-enzymatic step pathways and the importance of selecting an appropriate host for high-titre processes. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit; Ph.D. grant SFRH/BD/130739/2017 to Carlos E. Costa | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F130739%2F2017/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Lactose fermentation | por |
dc.subject | Constitutive promoters | por |
dc.subject | CRISPR/Cas9 | por |
dc.subject | Bioethanol | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.title | Strategic combination of different promoters in lactose metabolisation and host chassis selection for high bioethanol titres from dairy wastes | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/journal/bioresource-technology-reports | por |
dc.comments | CEB55607 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 8 | por |
oaire.citationVolume | 19 | por |
dc.date.updated | 2022-07-03T11:11:10Z | - |
dc.identifier.eissn | 2589-014X | por |
dc.identifier.doi | 10.1016/j.biteb.2022.101131 | por |
dc.date.embargo | 10000-01-01 | - |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Bioresource Technology Reports | por |
dc.identifier.articlenumber | 101131 | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55607_1.pdf Acesso restrito! | 6,66 MB | Adobe PDF | Ver/Abrir |