Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78478
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Carlos, Gabriela Soares Diogo | por |
dc.contributor.author | Marques, Catarina | por |
dc.contributor.author | Ribeiro, Sara Freitas | por |
dc.contributor.author | Sotelo, Carmen G. | por |
dc.contributor.author | Pérez-Marin, Ricardo I. | por |
dc.contributor.author | Pirraco, Rogério P. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Silva, Tiago H. | por |
dc.date.accessioned | 2022-06-22T15:30:20Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Diogo, G. S., Marques, C. F., Freitas-Ribeiro, S., Sotelo, C. G., Pérez-Martin, R. I., Pirraco, R. P., … Silva, T. H. (2022, February). Mineralized collagen as a bioactive ink to support encapsulation of human adipose stem cells: A step towards the future of bone regeneration. Biomaterials Advances. Elsevier BV. http://doi.org/10.1016/j.msec.2021.112600 | por |
dc.identifier.issn | 2772-9508 | por |
dc.identifier.uri | https://hdl.handle.net/1822/78478 | - |
dc.description.abstract | Bioprinting – printing with incorporated living cells – has earned special attention on tissue engineering approaches, aiming to closer reproduce the 3D microenvironment of the target tissue. However, it raises extra complexity related to the need to use cell-friendly printing conditions that still comply with material printing fidelity. Inspired by the composite nano structural organization of mineralized tissues, this work reports the efficiency of the chemical approach followed to in situ mineralize blue shark skin collagen, at a nano scale level, to ultimately produce stable inks. The influence of initial cellular density was evaluated by assessing three different concentrations (2.5, 5 and 7.5 × 106 cells·ml−1) of human adipose stem cells (hASC), with the higher density of encapsulated cells presenting improved viability in a long culture term. Immunodetection of osteogenic-related markers, like RUNX2 and osteopontin, 21 days after cell culture in basal conditions confirmed the potential of the ink to be applied for osteogenic purposes, which may be associated with the success of the cell-to-ink interaction and the Ca2+ ions released from the co-precipitated hydroxyapatite. A combination of mineralized shark collagen, alginate and hASC is thus proposed as a bioactive bioink with potential properties for regeneration of bone tissue. | por |
dc.description.sponsorship | This study was funded by the Portuguese Foundation for Science and Tecnology (FCT) under the scope of the BiogenInk project (M-ERA-NET2/ 0022/2016), by European Regional Development Fund through INTERREG Atlantic Area Programme, under the scope of BLUEHUMAN (EAPA_151/ 2016) project and through Norte de Portugal Regional Operational Program (NORTE 2020), under the scope of Structured project NORTE-01-0145- FEDER-000021. The Doctoral Programme NORTE-08-5369-FSE-000037 supported by NORTE 2020, under the PORTUGAL 2020 Partnership Agreement, through the European Social Fund, is also greatly acknowledge by the PhD fellowship of GSD. RP and SF-R thank FCT for the investigator contract IF/00347/2015 and PhD grant PD/BD/135252/2017, respectively. The authors acknowledge also the Centro Tecnológico del Mar (CETMAR, Vigo, Spain) and COPEMAR SA (fishing company, Spain) for the kind offer of the used by-products for the production of collagen. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/M-ERA-NET2%2F0022%2F2016/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00347%2F2015%2FCP1294%2FCT0004/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F135252%2F2017/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | 3D bioprinting | por |
dc.subject | Bone regeneration | por |
dc.subject | Co-precipitation | por |
dc.subject | Marine collagen | por |
dc.title | Mineralized collagen as a bioactive ink to support encapsulation of human adipose stem cells: a step towards the future of bone regeneration | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0928493121007402 | por |
dc.comments | http://3bs.uminho.pt/node/20786 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 13 | por |
oaire.citationVolume | 133 | por |
dc.date.updated | 2022-06-22T09:17:34Z | - |
dc.identifier.doi | 10.1016/j.msec.2021.112600 | por |
dc.date.embargo | 10000-01-01 | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomaterials Advances | por |
dc.identifier.articlenumber | 112600 | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20786-1-s20-s0928493121007402-main-1.pdf Acesso restrito! | 5,83 MB | Adobe PDF | Ver/Abrir |