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https://hdl.handle.net/1822/78436
Título: | Successive fermentation of aguamiel and molasses by Aspergillus oryzae and Saccharomyces cerevisiae to obtain high purity fructooligosaccharides |
Autor(es): | Flores, Orlando Flores-Gallegos, Adriana Carolina Muñíz-Márquez, Diana Contreras-Esquivel, Juan C. Teixeira, J. A. Nobre, Clarisse Aguilar, Cristóbal N. |
Palavras-chave: | Aspergillus oryzae whole-cell fermentation fructooligosaccharides low-cost media aguamiel molasses successive fermentation |
Data: | 17-Jun-2022 |
Editora: | MDPI AG |
Revista: | Foods |
Citação: | de la Rosa, O.; Flores-Gallegos, A.C.; Muñíz-Márquez, D.; Contreras-Esquivel, J.C.; Teixeira, J.A.; Nobre, C.; Aguilar, C.N. Successive Fermentation of Aguamiel and Molasses by Aspergillus oryzae and Saccharomyces cerevisiae to Obtain High Purity Fructooligosaccharides. Foods 2022, 11, 1786. https://doi.org/10.3390/foods11121786 |
Resumo(s): | Fructooligosaccharides (FOS) are usually synthesized with pure enzymes using highly concentrated sucrose solutions. In this work, low-cost aguamiel and molasses were explored as sucrose alternatives to produce FOS, via whole-cell fermentation, with an Aspergillus oryzae DIA-MF strain. FOS production process was optimized through a central composite experimental design, with two independent variables: initial sucrose concentration in a medium composed of aguamiel and molasses (AgMe), and inoculum concentration. The optimized process—165 g/L initial sucrose in AgMe (adjusted with concentrated molasses) and 1 × 107 spores/mL inoculum concentration—resulted in an FOS production of 119 ± 12 g/L and a yield of 0.64 ± 0.05 g FOS/g GFi. Among the FOSs produced were kestose, nystose, 1-fructofuranosyl-nystose, and potentially a novel trisaccharide produced by this strain. To reduce the content of mono- and disaccharides in the mixture, run a successive fermentation was run with two Saccharomyces cerevisiae strains. Fermentations run with S. cerevisiae S227 improved FOS purity in the mixture from 39 ± 3% to 61.0 ± 0.6% (w/w) after 16 h of fermentation. This study showed that agro-industrial wastes such as molasses with aguamiel are excellent alternatives as substrate sources for the production of prebiotic FOS, resulting in a lower-cost process. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/78436 |
DOI: | 10.3390/foods11121786 |
ISSN: | 2304-8158 |
Versão da editora: | https://www.mdpi.com/2304-8158/11/12/1786 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55599_1.pdf | 2,3 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons