Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78412
Título: | Identification of novel aptamers targeting cathepsin B-overexpressing prostate cancer cells |
Autor(es): | Pereira, Ana Cláudia Santos Pina, André F. Sousa, Diana Andrade Ferreira, Débora Carina Gonçalves Abreu Cátia S. Pereira Rodrigues, Joana Lúcia Lima Correia Melo, Luís Daniel Rodrigues Sales, M. G. F. Sousa, Sérgio F. Rodrigues, L. R. |
Data: | 17-Mar-2022 |
Editora: | Royal Society of Chemistry |
Revista: | Molecular Systems Design & Engineering |
Citação: | Pereira, A. C., Pina, A. F., Sousa, D., Ferreira, D., Santos-Pereira, C., Rodrigues, J. L., . . . Rodrigues, L. R. (2022). Identification of novel aptamers targeting cathepsin B-overexpressing prostate cancer cells. [10.1039/D2ME00022A]. Molecular Systems Design & Engineering, 7(6), 637-650. doi: 10.1039/d2me00022a |
Resumo(s): | Increased levels of cathepsin B (CatB), a cysteine protease, have been associated with different types of tumors, including prostate cancer. Hence, the identification of novel targeting ligands homing CatB may be a promising approach for the development of CatB-targeted therapies. In this work, a methodology called systematic evolution of ligands by EXponential enrichment (SELEX) was used to generate and isolate new aptamers targeting CatB. After 8 rounds of selection, the pool was sequenced, and the 10 most prevalent sequences, along with their corresponding reverse complementary sequences, were further analyzed. In order to assess the binding affinity of the aptamers towards the CatB, bioinformatics tools were used. After generating the 3D structures for each aptamer, the HADDOCK software was used for the docking studies between them and CatB. Molecular dynamics simulations and free binding energy calculations by molecular mechanics-generalized born surface area (MM-GBSA) allowed selection of a new aptamer with potential to target CatB. Cell binding assays against the PC-3 prostate cancer cell line confirmed the in silico predictions, further validating the newly discovered aptamer as a promising targeting ligand for CatB-overexpressing cancer cells. |
Tipo: | Artigo |
Descrição: | Supplementary files: https://www.rsc.org/suppdata/d2/me/d2me00022a/d2me00022a1.pdf |
URI: | https://hdl.handle.net/1822/78412 |
DOI: | 10.1039/D2ME00022A |
ISSN: | 2058-9689 |
e-ISSN: | 2058-9689 |
Versão da editora: | https://pubs.rsc.org/en/content/articlelanding/2022/ME/D2ME00022A |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55414_1.pdf Acesso restrito! | 4,32 MB | Adobe PDF | Ver/Abrir |