Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78219
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pinho, Diana | por |
dc.contributor.author | Faustino, Vera | por |
dc.contributor.author | Catarino, Susana Oliveira | por |
dc.contributor.author | Pereira, Ana I. | por |
dc.contributor.author | Minas, Graça | por |
dc.contributor.author | Pinho, Fernando T. | por |
dc.contributor.author | Lima, Rui Alberto Madeira Macedo | por |
dc.date.accessioned | 2022-06-03T09:36:15Z | - |
dc.date.available | 2022-06-03T09:36:15Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | D Pinho, V Faustino, SO Catarino, AI Pereira, G Minas, FT Pinho, R Lima, Label-free multi-step microfluidic device for mechanical characterization of blood cells: Diabetes type II, Micro and Nano Engineering, Volume 16, August 2022, 100149 (DOI: 10.1016/j.mne.2022.100149) | por |
dc.identifier.issn | 2590-0072 | - |
dc.identifier.uri | https://hdl.handle.net/1822/78219 | - |
dc.description.abstract | The increasing interest to establish significant correlations between blood cell mechanical measurements and blood diseases, has led to the promotion of microfluidic devices as attractive clinical tools for potential use in diagnosis. A multi-step microfluidic device able to separate red and white blood cells (RBCs and WBCs) from plasma and simultaneously measure blood cells deformability (5 and 20% of hematocrit) is presented in this paper. The device employs passive separation based on the cross-flow filtration principle, introduced at each daughter channel. At the outlets, hyperbolic geometries allow single-cell deformability analysis. The device was tested with blood from five healthy and fifteen diabetic type II voluntary donors. The results have shown that WBCs have lower deformability than RBCs, and no significant differences were observed in WBCs from healthy and pathological blood samples. In contrast, RBCs have shown significant differences, with pathological cells exhibiting lower deformability. Shear rheology has shown that blood from patients with type II diabetes has higher viscosity than blood from healthy donors. This microfluidic device has demonstrated the ability to reduce cell concentration at the outlets down to 1%, an ideal cell concentration for assessing the blood cells deformability, under healthy and pathological conditions. The results provide new insights and quantitative information about the hemodynamics of in vitro type II diabetes mellitus RBCs. Thus, such device can be a promising complement in clinical diagnosis and biological research as part of an integrated blood-on-a-chip system. | por |
dc.description.sponsorship | This work was supported by Projects NORTE-01-0145-FEDER-028178, NORTE-01-0145-FEDER-029394, NORTE-01-0145-FEDER-030171 funded by COMPETE2020, NORTE2020, PORTUGAL2020, and FEDER. This work was also supported by Fundacao para a Ciencia e a Tecnologia (FCT) under the strategic grants UIDB/04077/2020 and UIDB/00532/2020. D. Pinho and V. Faustino acknowledge the Ph.D. scholarships SFRH/BD/89077/2012 and SFRH/BD/99696/2014, respectively, both provided by FCT. Susana Catarino thanks FCT for her contract funding provided through 2020.00215.CEECIND. F. T. Pinho is thankful to FCT for financial support through projects LA/P/0045/2020 of the Associate Laboratory in Chemical Engineering (ALiCE) and pro-jects UIDB/00532/2020 and UIDP/00532/2020 of Centro de Estudos de Fenomenos de Transporte. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | NORTE-01-0145-FEDER-028178 | por |
dc.relation | NORTE-01-0145-FEDER-029394 | por |
dc.relation | NORTE-01-0145-FEDER- 030171 | por |
dc.relation | UIDB/04077/2020 | por |
dc.relation | UIDB/00532/2020 | por |
dc.relation | UIDP/00532/2020 | por |
dc.relation | SFRH/BD/89077/2012 | por |
dc.relation | SFRH/BD/99696/2014 | por |
dc.relation | 2020.00215.CEECIND | por |
dc.relation | LA/P/0045/2020 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | por |
dc.subject | Hydrodynamic separation | por |
dc.subject | Blood-on-a-chip | por |
dc.subject | Viscosity | por |
dc.subject | Deformability | por |
dc.subject | Diabetes type II | por |
dc.subject | Biomicrofluidics | por |
dc.title | Label-free multi-step microfluidic device for mechanical characterization of blood cells: diabetes type II | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S2590007222000466 | por |
oaire.citationIssue | August | por |
oaire.citationVolume | 16 | por |
dc.identifier.eissn | 1873-5568 | - |
dc.identifier.doi | 10.1016/j.mne.2022.100149 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.fos | Engenharia e Tecnologia::Engenharia Mecânica | por |
dc.subject.fos | Engenharia e Tecnologia::Engenharia Médica | por |
dc.subject.fos | Engenharia e Tecnologia::Outras Engenharias e Tecnologias | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Micro and Nano Engineering | por |
oaire.version | VoR | por |
dc.identifier.articlenumber | 100149 | por |
dc.subject.ods | Saúde de qualidade | por |
Aparece nas coleções: | CMEMS - Artigos em revistas internacionais/Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S2590007222000466-main.pdf | Publication | 2,64 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons