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https://hdl.handle.net/1822/7754
Título: | Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity |
Autor(es): | Oliveira, M. D. L. Andrade, C. A. S. Magalhães, N. S. Coelho, L. C. B. B. Teixeira, J. A. Cunha, M. G. C. Correia, M. T. S. |
Palavras-chave: | Antibacterial lectin Bacteria Eugenia uniflora Eugenia uniflora seed Lectin Lectin purification |
Data: | Mar-2008 |
Editora: | The Society for Applied Microbiology |
Revista: | Letters in Applied Microbiology |
Citação: | Oliveira, M. D. L., Andrade, C. A. S., Santos-Magalhães, N. S., Coelho, L. C. B. B., Teixeira, J. A., Carneiro-da-Cunha, M. G., & Correia, M. T. S. (2008, March). Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity. Letters in Applied Microbiology. Wiley. http://doi.org/10.1111/j.1472-765x.2007.02319.x |
Resumo(s): | Aims: The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds. Methods and Results: The E. uniflora lectin (EuniSL) was isolated from the seed extract and purified by ion-exchange chromatography in DEAE-Sephadex with a purification factor of 11·68. The purified lectin showed a single band on denaturing electrophoresis, with a molecular mass of 67 kDa. EuniSL agglutinated rabbit and human erythrocytes with a higher specificity for rabbit erythrocytes. The haemagglutination was not inhibited by the tested carbohydrates but glycoproteins exerted a strong inhibitory action. The lectin proved to be thermo resistant with the highest stability at pH 6·5 and divalent ions did not affect its activity. EuniSL demonstrated a remarkable nonselective antibacterial activity. EuniSL strongly inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella sp. with a minimum inhibitory concentration (MIC) of 1·5 μg ml−1, and moderately inhibited the growth of Bacillus subtilis, Streptococcus sp. and Escherichia coli with a MIC of 16·5 μg ml−1. Conclusions: EuniSL was found to be effective against bacteria. Significance and Impact of the Study: The strong antibacterial activity of the studied lectin indicates a high potential for clinical microbiology and therapeutic applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/7754 |
DOI: | 10.1111/j.1472-765X.2007.02319.x |
ISSN: | 0266-8254 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Oliveira_Lam[1].pdf | 149,15 kB | Adobe PDF | Ver/Abrir |