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https://hdl.handle.net/1822/77212
Título: | Purification of chitosanases produced by Bacillus toyonensis CCT 7899 and functional oligosaccharides production |
Autor(es): | Dantas, Julia Maria de Medeiros Araújo, Nathália Kelly de Silva, Nayara Sousa da Torres-Rêgo, Manoela Furtado, Allanny Alves Assis, Cristiane Fernandes de Araújo, Renata Mendonça Teixeira, J. A. Ferreira, Leandro de Santis Fernandes-Pedrosa, Matheus de Freitas Santos, Everaldo Silvino dos |
Palavras-chave: | Anti-inflammatory Chitooligosaccharides Chitosanases |
Data: | 2022 |
Editora: | Marcel Dekker Inc. |
Revista: | Preparative Biochemistry and Biotechnology |
Citação: | Dantas, Julia Maria de Medeiros; Araújo, Nathália Kelly de; Silva, Nayara Sousa da; Torres-Rêgo, Manoela; Furtado, Allanny Alves; Assis, Cristiane Fernandes de; Araújo, Renata Mendonça; Teixeira, José A.; Ferreira, Leandro de Santis; Fernandes-Pedrosa, Matheus de Freitas; Santos, Everaldo Silvino dos, Purification of chitosanases produced by Bacillus toyonensis CCT 7899 and functional oligosaccharides production. Preparative Biochemistry & Biotechnology, 52(4), 443-451, 2022 |
Resumo(s): | Chitooligosaccharides (COS) have a great potential to be used by pharmaceutical industry due to their many biological activities. The use of enzymes to produce them is very advantageous, however it still faces many challenges, such as discovering new strains capable to produce enzymes that are able to generate bioactive oligosaccharides. In the present study a purification protein protocol was performed to purify chitosanases produced by Bacillus toyonensis CCT 7899 for further chitosan hydrolysis. The produced chitooligosaccharides were characterized by mass spectroscopy (MS) and their antiedematogenic effect was investigated through carrageenan-induced paw edema model. The animals were treated previously to inflammation by intragastric route with COS at 30, 300 and 600mg/kg. The purification protocol showed a good performance for the chitosanases purification using 0.20M NaCl solution to elute it, with a 9.54-fold purification factor. The treatment with COS promoted a decrease of paw edema at all evaluated times and the AUC04h, proving that COS produced showed activity in acute inflammation like commercial anti-inflammatory Dexamethasone (corticosteroid). Therefore, the strategy used to purification was successfully applied and it was possible to generate bioactive oligosaccharides with potential pharmacological use. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/77212 |
DOI: | 10.1080/10826068.2021.1961273 |
ISSN: | 1082-6068 |
Versão da editora: | http://www.tandfonline.com/action/journalInformation?journalCode=lpbb20 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54595_1.pdf Acesso restrito! | 1,94 MB | Adobe PDF | Ver/Abrir |