Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/76693
Título: | Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
Autor(es): | Bispo, Mafalda Santos, Sílvio Roberto Branco Melo, Luís Daniel Rodrigues Azeredo, Joana Dijl, Jan Maarten van |
Palavras-chave: | Cell-binding domain Endolysin Antimicrobial photodynamic therapy Intracellular Staphylococcus aureus Staphylococcus epidermidis Staphylococcus aureus Biofilms Intracellular bacteria |
Data: | 23-Mar-2022 |
Editora: | American Society for Microbiology (ASM) |
Revista: | Microbiology Spectrum |
Citação: | Bispo, Mafalda; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Azeredo, Joana; Dijl, Jan Maarten van; Rosato, Adriana E., Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain. Microbiology Spectrum, 10(1), e01466-21, 2022 |
Resumo(s): | Antimicrobial resistance is among the biggest threats to mankind today. There are two alternative antimicrobial therapies that may help to control multidrug-resistant bacteria. In phage therapy, natural antagonists of bacteria, lytic phages, are harnessed to fight pathogens. In antimicrobial photodynamic therapy (aPDT), a photosensitizer, molecular oxygen, and light are used to produce reactive oxygen species (ROS) that inflict lethal damage on pathogens. Since aPDT destroys multiple essential components in targeted pathogens, aPDT resistance is unlikely. However, the challenge in aPDT is to maximize target specificity and minimize collateral oxidative damage to host cells. We now present an antimicrobial approach that combines the best features of both alternative therapies, namely, the high target specificity of phages and the efficacy of aPDT. This is achieved by conjugating the specific cell-binding domain from a phage protein to a near-infrared photosensitizer. aPDT with the resulting conjugate shows high target specificity toward MRSA with minimal side effects. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/76693 |
DOI: | 10.1128/spectrum.01466-21 |
ISSN: | 2165-0497 |
Versão da editora: | https://journals.asm.org/journal/spectrum |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55419_1.pdf | 408,05 kB | Adobe PDF | Ver/Abrir |