Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/76422
Título: | Orotic acid production from crude glycerol by engineered Ashbya gossypii |
Autor(es): | Silva, Rui Aguiar, Tatiana Quinta Domingues, Lucília |
Palavras-chave: | De novo pyrimidine biosynthesis Ashbya gossypii Saccharomyces cerevisiae URA9 Dihydroorotate dehydrogenase Orotic acid Orotic acid (PubChem CID: 967) |
Data: | Fev-2022 |
Editora: | Elsevier 1 |
Revista: | Bioresource Technology Reports |
Citação: | Silva, Rui; Aguiar, Tatiana Q.; Domingues, Lucília, Orotic acid production from crude glycerol by engineered Ashbya gossypii. Bioresource Technology Reports, 17(100992), 2022 |
Resumo(s): | Orotic acid is an intermediate of the de novo pyrimidine biosynthesis that plays a crucial role in several nutraceutical supplements. In some microorganisms, blockage of this pathway downstream the dihydroorotate dehydrogenase (DHOD) leads to orotic acid accumulation. Here, the Ashbya gossypii Agura3 pyrimidine auxotroph was shown to accumulate and excrete orotic acid (~1.5g/L), and this trait was further explored for the production of orotic acid from raw substrates. Metabolic engineering of this strain combined with culture conditions optimization led to a 3.6-fold increase in orotic acid production from crude glycerol. The mitochondrial DHOD encoded by AgURA9 was shown to be determinant for this phenotype. Heterologous expression of AgURA9 in engineered S. cerevisiae enabled the accumulation and excretion of orotic acid (~0.5g/L). This study demonstrates the potential of A. gossypii for the valorization of crude glycerol to orotic acid and discloses the molecular determinants for its biosynthesis in fungi. |
Tipo: | Artigo |
Descrição: | Supplementary data to this article can be found online at https://doi. org/10.1016/j.biteb.2022.100992. |
URI: | https://hdl.handle.net/1822/76422 |
DOI: | 10.1016/j.biteb.2022.100992 |
ISSN: | 2589-014X |
Versão da editora: | https://www.sciencedirect.com/journal/bioresource-technology-reports |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_55322_1.pdf | 617,26 kB | Adobe PDF | Ver/Abrir |