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https://hdl.handle.net/1822/75578
Título: | Hyaluronic acid oligomer immobilization as an angiogenic trigger for the neovascularization of TE constructs |
Autor(es): | Silva, A. L. Babo, Pedro Miguel Sousa Rodrigues, Márcia T. Gonçalves, A I Novoa-Carballal, Ramon Pires, R. A. Rouwkema, J. Reis, R. L. Gomes, Manuela E. |
Palavras-chave: | Angiogenesis Hyaluronic acid Methacrylated gelatin Tissue engineering Acid oligomer Vascularization Angiogenesis Dental pulp regeneration hyaluronic acid oligomers |
Data: | Jul-2021 |
Editora: | American Chemical Society |
Revista: | ACS Applied Bio Materials |
Citação: | Silva, A. L., Babo, P. S., Rodrigues, M. T., Gonçalves, A. I., Novoa-Carballal, R., Pires, R. A., ... & Gomes, M. E. (2021). Hyaluronic Acid Oligomer Immobilization as an Angiogenic Trigger for the Neovascularization of TE Constructs. ACS Applied Bio Materials, 4(8), 6023-6035 |
Resumo(s): | Tissue engineered (TE) substitutes of clinically relevant sizes need an adequate vascular system to ensure function and proper tissue integration after implantation. However, the predictable vascularization of TE substitutes is yet to be achieved. Molecular weight variations in hyaluronic acid (HA) have been pointed to trigger angiogenesis. Thus, this study investigates HA oligomer immobilization as a promoter for TE construct vascularization. As a proof-of-concept, the surface of methacrylated gelatin (GelMA) hydrogels were functionalized with high molecular weight (HMW; 1.5 to 1.8 MDa) and low molecular weight (LMW; < 10 kDa) HA, previously modified with aldehyde groups to enable the immobilization through Schiffâ s base formation. The ability of A-HA to bind amine-presenting surfaces was confirmed by Surface Plasmon Resonance (SPR). Human Umbilical Vein Endothelial Cells (HUVECs) seeded over hydrogels functionalized with LMW HA showed higher proliferation and expression of angiogenic markers (KDR and CD31), than those grown in HMW HA conjugated- or plain surfaces, in line with the activation of HA ERK1/2 mediated downstream signaling. Moreover, when cocultured with human dental pulp cells (hDPCs) encapsulated into the GelMA, an increase in endothelial cell migration was observed for the LMW HA functionalized formulations. Overall LMW HA functionalization enhanced endothelial cell response showing potential as an angiogenesis inducer for TE applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75578 |
DOI: | 10.1021/acsabm.1c00291 |
ISSN: | 2576-6422 |
Versão da editora: | https://pubs.acs.org/doi/abs/10.1021/acsabm.1c00291 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20699-alsilva-acs-apl-bio-mat-2021.pdf | 1,48 MB | Adobe PDF | Ver/Abrir |